| In order to building high-performance of Ampelopsis grossedentata (Hand-Mazz) W.T.Wang breeding system, this paper suffered Ampelopsis grossedentata stem and leaf of the Hunan Agricultural University Resources garden, for the material, about 10 to 15 stems and 1 to 5 stems for stem explants (referred to as P1, P2), to cut off the tip and the following 2/3 of leaf petiole of the leaf with the leaflets as explants (referred to as P3). To study the factors affecting of plant regeneration, the results are as follows:1. The Optimal disinfecting programs of Ampelopsis grossedentata tissue culture are as follows:the stem and leaf materials immerse in 75 percent ethanol about 10 seconds, then P1 use 0.25% HgCl2 disinfecting 25 minutes, P2 and P3 use 0.15% HgC12 disinfecting 15 minutes.2. Callus induction of P3 is in BA 2.0mg/L and IBA0.05mg/L with 20% sucrose of MS medium, and callus induction of P1,P2 is in BA1.5 mg/L and IBA 0.05 mg/L with 20% sucrose of MS medium. Callus induction of P1,P2 differentiate the abnormal embryos, but failed to further differentiating the bud.3.Stem segments with axillary bud explants in culture, only induced the growth of axillary buds, do not produce adventitious buds. The germination rate of axillary bud with high lignification degree is superior to low lignification degree.Stem explants with non-axillary bud and axillary bud basal cells are easy to induce multiple shoots.Low lignification explant shoot induction rate was significantly higher than the high degree of lignification explants.4.Leaf explants with leaflets petiole cultured in the BM medium add TDZ 0.1 mg/L, IBA 0.5 mg/L and 2% sucrose, induced the adventitious buds5. The Plantlets stems with mature leaves in the. MS+IBA 0.05 mg/L culture medium induced more easily take root.6.The Ampelopsis grossedentata tube seedlings cuttings in the soil with the mass ratio Wong Nai to sand is 2 to 1, temperature 25℃, humidity over 75%. The survival rate is 93%.
|
PDF Full Text Request