| Development of new fertility restorer lines with pyramided good quality and disease resistances is very important for breeding hybrid wheat. 2114 is a restorer line containing a restore gene, Rf6, derived from Aegilops umbellulata zhuk. However, 2114 is sensitive to powdery mildew. 92R149, a wheat-H. villosa 6VS/6AL translocation line, contains a powdery mildew resistance gene, Pm21. In orden to develop new restorer lines with powdery mildew resistance, the line 2114 was crossed to 92R149, and a new fertility restorer line with powdery mildew was identified from the F6 progenies by mitotic and meiotic analysis combined with chromosome C-banding, bi-color fluorescence in situ hybridization(FISH) and molecular marker analysis. This new fertility restorer line was identified to be a double translocation of 6AL·6VS, 6BS·6BL-6US. After inoculated with a local Blumeria graminis mixture at the four-leaf stage in a greenhouse of the Jiang Pu experimental station, Nanjing Agricultural University, this new restorer line showed high resistance to powdery mildew, with similar resistance level as 92R149. In order to further evaluate the restoring ability of the new restorer line, a sterile line ND35 was used as female to cross with the new fertility restorer line, and the data from the F1 hybrid indicated that the restoring ability of the new fertility restorer line was more than 90％in average both in the greenhouse and field conditions.
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