Study On Separation Flavonoids From Pomelo

The grapefruit is the"YUN XIANG"spice orange belong to the fruit which not only have the higher nourishment value,but also have the very high medicine value.According to the measurement,grapefruit not only implying the healthy essential nourishment composition,such as various amino acids,carotenes of various human bodies,but also have the prevention and cure function to some diseases to the human bodies.the namely so-called plant chemistry composition(photochemical).The grapefruit skin imply the volatile oil,etc.Which have the anti- to oxidize the live function and these compositions are obvious high in the content in the fruit peel of grapefruit.Therefore,the fruit peel of grapefruit has the higher health care and medicines to use the value.The falconoid compound has the extensive application on the food and the medicine industries can used to the anti- oxidizing agent and the raw material of the function foods on the food industry.Local mainly withdraw the raw material of the Flavonoids to spend for the"huaihua"currently,the resources is more rarely seen,can't satisfy the market need,so the full development makes use of the sleeves skin resources to have very important realistic meaning.This research used the hull of pomelo as raw material approached the method of separation and purification.The results showed as follows:1.The Determination of falconoid that is in pomelo was studied by means of high performance liquid chromatography(HPLC).The HPLC system was consisted of ODS C18(4.6mm×250mm,5μm)and UV detector set at 280nm.The mobile phase was V (CH3OH):V(H2O)=35:65 The experiment showed that this method is sensitive, accurate and reliable.2.The extraction technology of flavonoids was selected by HPLC which mensurate the content of total pomelo as an index.the optimum extraction condition of ultrasound was determined by orthogonal design method and variance analysis as follows.The power of ultrasonic was 100w,ratio of raw materials and solution as 1:5,time was 45 min,three extraction time.3.Seperation and Purification is the important step in obtaining effective ingredients from natural material.The experiment results showed that a resin type of X-5 was better in adsorption and desorption to flavonoids.Flaavonoids can be purified well under the condition of PH 6,material concentration 0.3g/ml,sample flow velocity 1.0ml/min, material qulity 7ml.