Screening And Primarily Analysis The Differential Expression Genes Related To The Development Of Goose Fatty Liver

In order to search for the molecular mechanism of the goose fatty liver, 12 Landes geese were used in this study. These geese were divided into two groups and 6 geese in each group. The differential expression bands between overfeeding group and control group were detected with technique of mRNA differential display. These differential expression bands were cloned and sequenced and found homologous genes through comparison with known genes from the GenBank, and the possible functions should be predicted. In addition, because of the closely correlation between fat metabolism and development of goose fatty liver, 7 candidate genes of fatty trait were involved in the present study, such as: Adipocyte fatty acid binding protein (A-FABP) gene, liver fatty acid binding protein (L-FABP) gene, proxisome proliferators-activated receptor alpha and gmma gene (PPARαand PPARγ) gene, lipoprotein lipase(LPL)gene, liver X receptor(LXRs) gene and adiponectin(AdipoQ) gene. The mRNA level of these candidate genes and new differential expression genes in goose fatty liver were detected with technique of semi-quantitative RT-PCR, and their effect on the development of fatty liver were explored. The results are as follows:(1) 40 differential expression bands were found between overfeeding group and control group using 90 pairs primer combination, and 22 differential expression sequences were obtained successfully through recovering bands, twice PCR, cloning and sequencing. From the 22 differential expression bands, 8 bands that have homologization highly with known functional genes were found; 6 bands were similar with known EST or cDNA, but their functions were unknown; other 8 bands were new EST, their homologies were not found in GenBank.(2) 8 differential expression genes whose functions are known, are fibrinogen alpha chain(FAG) gene, fibrinogen gamma chain(FGG) gene, Chromobox homolog 6(CBX6) gene, RNA binding motif protein 7(RBM7), transmembrane protein 53(TMEM 53), matrix metallopeptidase-11 (MMP-11 ) gene, C18orf1 gene and zinc finger protein 469(ZNF469 ) gene.(3) With the combination of the DDRT-PCR and semi-quantitative RT-PCR analysis, the following genes were significantly up-regulated in goose fatty liver: FGA, CBX 6, TMEM 53, MMP-11, ZNF469, A-FABP, L-FABP and PPARγ, while 5 genes including FGG, RBM7, C18orf1, PPARαand LPL gene were down-regulated in goose fatty liver. Additionally, the difference of LXRαexpressed between overfeeding group and control group was not significant, and AdipoQ was not expressed in the two groups.(4) According to the previous studies, these genes were involved in the fat metabolism, tumorous transfer, RNA modify and regulation. Induced by the high-energy feeds, the special changes of the expression of these genes in goose fatty liver may be important factors in promoting the fatty accumulation in goose fatty liver. So these genes may be the candidate genes of the development of goose fatty liver.