Effect Of Different Fatty Sources On Fatty Liver Performance And Gene Expression Of FAS MRNA In Landes Goose

450 healthy Landes geese of 70 days old with similar body weight were randomly assigned into 3 groups, each with 4 replicates of 50 birds according to the single factor experiment design. The first group was control group, others were experimental groups. In addition to overfeed, 2% of goose oil , 2% of bean oil and 2% of corn oil were added into the first, the second and the third group respectively. The effect of different fatty sources on fatty liver performance, fat deposition out of liver and nutritional components was investigated after three weeks of overfeed, and the expressed level of FAS mRNA in liver were detected and correlated by the real-time quantitative PCR , and the formation mechanism of goose fat liver were studied further on the molecular level .The results were as follows:1. 2% of oil were added into diets, without significant difference in each abatage index (except the increased weight in force-feeding) of the experimental group compared with the control group(P>0.05), The experimental guoup gained much more weight than the control group, with obvioius significance(P>0.05); The liver weight of each group were 709.28 g,824.64 g,808.82g respectively, which were 8-9.4 times before feeding. There were significant difference between experimental group and control group.(P<0.05). The liver weight of bean oil group and corn oil group were 16.26% and 14.03% higher than the goose oil group, it showed no significance between bean oil group and corn oil group(P>0.05). After the feeding, the liver-body ratio and the liver-dead body ratio increased significantly to compare with before feeding (P﹤0.05), it showed no significance between bean oil group and corn oil group(P>0.05), The feed-live ratio of experimental groups were not signifant(P>0.05, the bean oil group were the lowest. The best body gain and liver gain group were the bean oil group during feeding period.2. After force-feeding, With significant difference in abdominal fat weight and abdominal rate of the experimental group compared with the control group(P﹤0.05),it showed no significance between bean oil group and corn oil group (P>0.05).Without significant difference in sebum thick of the experimental group compared with the control group(P>0.05).3. After the force-feeding, the density of TG,HDL,LDL and CHOL in blood serum of experimental group were significantly lower than the control group(P<0.05), it showed no significance between bean oil group and corn oil group(P>0.05).4. After force-feeding, it shows no significant difference in water, protein, fat, lecithin, cephalin or unsaturated fatty acid in liver compared with control group(P>0.05),with significant difference compared with control group(P﹤0.05). However, the major constituent of liver changed significantly after the feeding: fat content increased more than 7 times, lecithin increased 8-10 times, cephalin increased 18-26 times, unsaturated fatty acid increased 2.5-3.5 times, while water and protein decreased 3 times and 2 times respectively.5. After force-feeding, water in breast muscle of the experimental group increased significantly(P﹤0.05), There were significance between bean oil group and corn oil group(P>0.05), but were 4.92% and 4.23% lower than the goose oil group; The protein content difference were not significant between experimental groups and control groups(P>0.05). The fat content of experimental groups increased significantly compared with the control group(P﹤0.05). The bean and the oil were not significant, increasing 4.58% and 4.09% compared with goose oil group(P>0.05).Without significant difference in protein content and amino acid of the experimental group in breast muscle compared with control group(P>0.05).6. 2% of oil were added into diets, Gene expression of FAS mRNA/β-actin mRNA increased significantly(P﹤0.05), but not significant among the trial groups(P>0.05), bean oil group>corn oil group>goose oil group.7. There was a positive genetic correlation between FAS mRNA expression in liver and the increased weight in force-feeding,liver weight,liver-body ratio and liver-dead body ratio(R=1.000,R=0.999,R=1.000 and R=1.000,respectively;P﹤0.05) Conclusion: 2% of oil were added into diets. Increased weight in force-feeding and fat liver performance of the vegetable oil group increased significantly, The bean oil group was the best and should be as the major oil source. The detectional results of each group for liver nutritional ingredients showed: after overfeed, content level of fat, lecithin, cephalin and unsaturated fatty acid in liver for each group were significantly higher, but content of water and protein were much lower, But it showed no significance among oil sources for the contribution to liver nutrition (excluding linoleate). Vegetable oil and fat can remarkably degrade water content in muscles, raise fat content, While there were no significant impact for protein content or amino acid content in muscle by different oil resources.The FAS mRNA expression in Landes liver was significantly influenced by different oil sources. Vegetable oil can remarkably raise the mRNA expression quantity,Among which the bean oil group were the highest. FAS mRNA expression in liver were positively correlated with increased weight in force-feeding,liver weight,liver-body ratio and liver-dead body ratio.It was suggested that the FAS gene plays a more important role during the body fat deposition and the formation of goose fat liver.