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Effects Of Transgenic Cry1Ab Rice On Growth And Development As Well As Symbiotic Microorganism Of The Brown Planthopper, Nilaparvata Lugens St(?)l

Posted on:2009-04-12Degree:MasterType:Thesis
Country:ChinaCandidate:X Y GaoFull Text:PDF
GTID:2143360242994383Subject:Agricultural Entomology and Pest Control
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The biosafety of transgenic crops has been becoming a focus in the world. Concerns of biosafety of transgenic rice are necessary, because rice is one of major foods of human. The effect of transgenic cry1Ab rice on the development and symbiotic microorganism of brown planthopper were studied and discussed in this paper.1 Effects of transgenic cry1Ab rice on the development of the brown planthopper.Raise the brown planthopper on transgenic cry 1 Ab rice in succession, and then studied the effect of transgenic cry1Ab rice on the development of brown planthopper. When brown planthopper feed on KMD1 and KMD2, there is no significant effect among the whole development of male or female with the brown planthopper feed on Xiushui 11. Transgenic cry1Ab rice KMD1 and KMD2 have no adverse effect on the development of brown planthopper.2 The effect of transgenic cry1Ab rice on yeast-like endosymbiote in abdomen fat body of brown planthopper.The quantity variety of yeast-like endosymbiote of brown planthopper shows that the number of yeast-like endosymbiote in female is descendent but ascending in male after feed on the transgenic cry1Ab rice. See from the generations, the number of yeast-like endosymbiote in female or male, there is no prominent difference from the first to the fifth generation after feed on KMD1, but have very prominent difference after feed on KMD2 and Xiushui 11. However, the quantity variety current of yeast-like endosymbiote of brown planthopper feed on KMD1 and KMD2 are same with the brown planthopper feed on Xiushui 11, therefore transgenic rice KMD1 and KMD2 have no adverse effect on the yeast-like endosymbiote of brown planthopper.3 The effect of transgenic cry1Ab rice on the diversity of intestinal microbial community in the brown planthopper by Biolog method.The diversity of intestinal microbial community in the brown planthopper, Nilaparvata lugens between fed on transgenic cry1Ab rice (KMD1 and KMD2) and on the non-transgenic control (Xiushui 11) was compared using Biolog-Eco method. The laboratory results showed that the diversity indexes including Shannon index, Simpson index and McIntosh index of the intestinal microbial community in the brown planthopper fed on KMD1 and KMD2 from the first to fifth generation were not significantly different from those fed on the control. In contrast, the average well color development (AWCD) of the intestinal microbial community in the brown planthopper fed on KMD1 was markedly higher than that fed on either KMD2 or the control. As to the field test, the result was almost as the same as that of the laboratory tests. In conclusion, it is suggested that effects of KMD1 and KMD2 on the diversity of the intestinal microbial community of non-target insect pest, Nilaparvata lugens were not significantly negative.
Keywords/Search Tags:Transgenic rice, cry1Ab, Nilaparvata lugens St(a|°)l, Dvelopment, Yeast-like endosymbiote, Biolog, Intestinal microbial, Diversity, Average well color development
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