| Kalanchoe blossfeldiana V.Poelln.are Crassulaceae.Kalanchoe Adans.perennial succulent of flowers and foliage plants..It occupys an important position on potted flowers and becomes one of the fastest-growing flower in international flower market.K.blossfeldiana V.Poelln.research so far is mainly on cultivation,propagation techniques and organizational aspects of culture in China.In foreign countries,most of the species remain in the types of physiological factors and contents of research,but the there is not any reports of tube micrografting.The species we have can not satisfy people's request,and micrografting is an utility way.At present,there is no report about effects to tube micrografting of K.blossfeldiana V.Poelln.In this experiment,I choosed C3(violet plentiful)and C6(unknown)of six K.blossfeldiana V. Poelln.varieties(C1～C6)which leaves form and flowers color are different from each other,C3 for the scion and C6 for stock.Useing the established sterile implant system to research with grafting method,cultivation conditions,deal with stock and scion and etc.And results were as the following:By using the establishment of a sterile implant system,we can breed fast,but unsuitable for stocks.Put C6 regenerate plants in MS + GA0.5mg/L + BA1.0mg/L culture medium for 30d,we get the adventitious buds with section between the elongation be uniform,average stem diameter increased to 0.179cm,and average height is 7.6 cm that could be suitable for the required stocks.Using cleft grafting and top grafting method,and the result sign:the top grafting method's survival rate is 73.3%,but the cleft grafting method's survival rate is only 33.3%.Top grafting method is the best.Put different density hormone in MS medium and the result sign:IAA is disadvantage to the plantlets.And MS+BA1.0mg/L+NAA0.1mg/L is the best medium for increasing the survival rate of grafted plantlets and its rapid growth.The survival rate is 100%,average height is 1.1cm, average growth rate is 0.009cm/d.Cultivate the plantlets in dark for 10d,15d and 20d.The result sign:dark for 20d,survival rate is 0%;dark for 10d,survival rate is only 63.3%;both light cultivation and dark for 10d,survival rate are 100%,but the plantlets growth fast with dark for 10d.When pH is 5.0 to 5.8,the survival rate is sublime and when pH is 5.8 to 6.5,the survival rate is breakdown.And the survival rate is highest 93.3%when pH is 5.8.To set up density of carrageenin with 5.6g/L,5.8g/L,6.0g/L,6.2g/L,6.4g/L,6.6g/L and 6.8 g/L.Plantlets is deformed and missing the value when the density is less than 5.8g/L.When it is greater than 6.4g/L,plantlets is chlorisis and wilting.The survival rate is highest 90%and plantlets growth good with density is 6.0g/L,so 6.0g/L is the best density of carrageenin.Put the plantlets in 15℃,20℃,25℃and 30℃.And 25℃is the optimum temperature with survival rate to be 93.3%.Double seeling membrane is better for plantlets' growing and survival rate is 93.3%.For improving micrografting survival rate,both of scions and rootstock should keep leaves, and survival rate is 73.3%.The effects of scions and stocks' length to survival rate would be not obvious,and the highest survival rate is 86.7%.The optimum rooting medium is 1/2MS+ IBA0.1mg/L +NAA0.1mg/L for micrografting plantlets of K.blossfeldiana V.Poelln.,the rooting rate is 83.3%and growing strong with 2.8cm long.Transplant 30 grafting plantlets to culture soil with Peat:Garden soil:Perlite = 4:4:2.After 20d,the survival rate is 83.3%.
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