Study On Influence Factors Of In Vitro Flowering Induction Of Achimenes Spp. And Kalanchoe Blossfeldiana

The common way to induce in vitro flowering is to induce flower buds differentiation from complete test-tube plantlet,which can bloom in any season in the test tube,avoiding the limitation of potted and cut flowers' florescence.It also preserves dynamic beauty which the so called immortal flower lack of it.These advantages of tube flowers meet the diverse needs of people for flower ornamental.Simultaneously,the morphological integrity and less time of floral bud induction make it play an important role both in variety exchange and in hybridization breeding research.Another way of in vitro flowering is to induce buds directly from explants,which presents a great potential for the study of bud formation mechanism.In the study,a reliable and efficient in vitro flowering protocol of two kinds of popular potted flowers:Achimenes spp.and Kalanchoe blossfeldiana were developed.The research mainly focused on the following parts,including plantlet strengthening,different ways of in vitro flower inducing and the main influencing factors of each way,the reasons and solutions of in vitro flowers failed to bloom,and flowering plant shape adjustment.The main results were as follows:1)Suitable medium for seedling strengthening of Achimenes spp.was selected.Plantlets were strong and leaf stretched well on the medium 1/2MS+NAA0.1mg/L+IBA0.3mg/L+active carbon 0.2%,adding CCC 2.0mg/L(for 'Kim blue')or CCC 1.0mg[L(for 'Aries')individually.2)A reliable and efficient in vitro flowering induction system of Achimenes spp.was establishmented.?The culture environmnet was 23±2?,light intensity 2500-3000lux,illumination time 14h/d.?Suitable medium for inducing in vitro flowering was:MS(KNO3+KH2PO4)+NAA0.1mg/L+IBA0.1mg/L+sucrose4%+agar0.4%+active carbon0.1%,adding PP3330.05mg/L and CCC 2.0mg/L('Kim blue')or PP3330.2mg/L and CCC1.0mg/L('Aries')individually.?Plantlets with 4 or more sections of shoot stem were necessary for flower buds induction.In this system,time for buds induction was 45d and buds number for each variety was 2.62 and 1.49 per pantlet,which could bloom for 12 to 15 days.3)Using unopened flower buds with 3mm long pedicel of K.blossfeldiana as explants,regenerated flower buds could be induced directly from it after 14 days of dark treatment,for which the suitable medium was:MS+6-BA0.5mg/L+NAA0.3 mg/L.4)Suitable medium for seedling strengthening of K.blossfeldiana was selected,which was 1/2MS+NAA0.1mg/L+IBA0.3mg/L+lactalbumin hydrolysate 0.05%+active carbon 0.4%,adding CCC 3.0mg/L('Nando' and 'Amarillo')or CCC2.0mg/L('Taranta red')individually.5)A reliable and efficient in vitro flowering induction system of K.blossfeldiana was establishmented.?The culture environmnet was 18±2?,light intensity 40001ux,illumination time 8h/d.?Suitable medium for inducing in vitro flowering was:MS+6-BA1.0mg/L+NAA0.1mg/L+IBA0.3mg/L+sucrose6%,adding PP3330.2mg/L('Nando' and 'Amarillo')or PP3330.1mg/L('Taranta red')individually.Given shorter than 8 hours light per day and plantlets with 4 section of shoot stem was necessary for flower bud differentiation.In this system,time for buds induction was 40d and average buds number was 25 to 35 per pantlet,which could bloom for 20 to 25 days.6)Adding CCC1.0mg/L and CCC2.0mg/L in the flower bud inducing medium for 'Taranta Red'and 'Amarillo' individually and remove the vessels from the short day environment 5 days after bud appearance could compact the flowering plants shape of K.blossfeldiana.