Study On The Phylogeny Of Seven Genera Of Diaspidinae Based On EF-1a Sequences

The Diaspidinae is Hemiptera, Diaspididae. Most of them can harm agricultural and forestry plants, fruit trees, flowers. Diaspididae as a kind of important quarantine pests has been of great concern. Therefore, studying the taxonomy has important economic significance.In this study,the phylogenetic relationships of the seven genera of Diaspidinae have been analyzed by using nuclear EF-1a sequences from 7 genera consisting of 13 species and other two outgroups in China. The base frequency,base substitution and genetic distance of DNA sequence have been analyzed by using some biosoftwares such as: DNAman,clustalX1.83,MEGA4.0 and PAUp*4.0bl0(Win) and so on. Phylogenetic Information has been tested via base substitution saturation analysis and PTP tests.Phy1ogenetic trees have been constructed with three methods , maximum parsimony(MP), neighbor-joining(NJ) and maximum likelihood (ML) on the software PAUP*4.0b10(win) and MEGA 4.0.The results are as follows.1.The average content of T,C,A and G of EF-1a of seven genera of Diaspidinae are 23.5%,25.6%,25.5% and 25.4% respectively. A+T content is 49% while G+C content is 51%;2. The transition (si) are 1.1 times than the transversion (sv) of seven families of Diaspidinae. Ti substitution mainly occurs between A and G and tv usually occurs between A and T. Substitution saturation analysis shows that the sequence is conservative;3. All the phylogenetic trees (NJ,MP and ME) show essentially the same topology,And that the lineages and the sublineages on the trees are linked tightly consistent with the taxonomically defined families and genera based on morphologies;4. Results also indicate that EF-1a is a useful molecular marker for Phylogenetic analysis at the family and genus level.The main results obtained in this study will be helpful in constructing standard system for morphological and molecular taxonomy and identifieation of Diaspidinae, and It will be very useful on taxonomy and identifieation of Diaspidinae species occurring in China.