The Breeding Of Kenaf Male Sterility And ISSR Maker Of Male Sterile Gene

Kenaf male sterility breeding gave a new method for the use of kenaf heterosis . The maker of genic-male sterility genes in kenaf is used in the chosen of good sterile line or maintainer line .Using per-plant back crossing to choose Sterile line and Using BSA to screen ISSR primer.Using plant AT-1found by kenaf breeding research group of institute of Bast fiber crops Chinese academy of agricultural sciences in san ya of hai nan province were used as female parent in crossing with 15-4, 04K1 and then backcrossed four times with male parent to select four steady sterile male sterility and maintainer line.Through surveying the kenaf backcross groups and F2 separate group found that the kenaf male sterility is controlled by major gene and the common gene.In the backcrossing groups we found some kenaf is part of the phenomenon infertility.It was indicated major gene is modificated by multiple sites. F2 isolated groups of fertility survey showed that the separation of male sterility Kenaf is 3:1, with MendelianThe DNA of every plant was extracted by CTAB to constitute the gene pool by BSA.Applying the ISSR technique we analysised the two gene pool.Finally we found a primer which can show the difference between the sterile gene pool and fertile gene pool.The ISSR primer is U859 . The result showed the male sterility is cytoplasmi nuclear interaction male sterile ,male sterility steady,and the ISSR primer U859 is linked with genic-male sterility...