Study On The Effect Of Insulin And Glucagons For The Abundances Of ADPN MRNA And HSL MRNA In Bovine Adipocyte

This is a trial about adding hormone in vitro. The greater omental adipocytes of neonatal calves were cultured by the method of adipocyte culture reported by previous literature. The effects of bovine insulin (0,5,10,20,50,100nmol/L)and bovine glucagon(0,25,100,200,500,1000pg/ml)on expressions of ADPN mRNA and HSL mRNA were detected by fluorescent quantitative PCR. The results showed that the expression level of HSL mRNA in adipocyte gradually decreased with the increase of INS concentration ranging from 5nmol/L to 100nm/L in culture media, when the INS concentration was over 20nmol/L, the depression effect became more obvious. It indicated that the expression of HSL mRNA depended on the dose of INS. The expression level of HSL mRNA in adipocyte gradually increased with the increase of glucagon concentration ranging from 25pg/mL to 1000pg/mL in culture media, especially, when the glucagons concentration was over 25pg/mL, the effect was more significant. The inhibition effect of INS on the expression of ADPN depended on the dose of INS. Higher dose of glucagon (≥500pg/ml) promoted the expression level of ADPN mRNA significantly (P<0.01), and the effect depended on the dose. These results indicated that INS and glucagon was likely to control the fat mobilization by regulating the expressions of ADPN mRNA and HSL mRNA in fat tissues.