Study On Polyamines Roles In Somatic Embryogenesis In Picea Mongolica(W.D.Xu)

The physiological and biochemical changes were studied during somatic embryogenesis in Picea mongolica (W.D.Xu) mature zygote embryo, and roles of polyamines on somatic embryogenesis and the mechanism were investigated.1. The conditions for somatic embryogenesis in Asparagus were optimized. The most suitable medium were: SH+2,4-D(0.1㎎/L)+6-BA (0.1㎎/L)for primary calluses induction and LS+ABA(5.0 mg/L)+PEG4000(50.0g/L) for embryogenic calluses induction. Whitish-yellow, bright, fresh, incompact and coincident particle embryogenic calli were obtained on medium supplemented with 50.0 g/LPEG4000 putrescine. Embryogenic calli can be induced within 60 days, and calli need be transfered to fresh medium within 50 days for subculture.2. Our test result showed that: The SOD activity is high, but the POD activity is low in process of embryogenic calli somatic embryogenesis in Picea mongolica(W.D.Xu).The POD enzyme activity is rising,the SOD activity is reducing when add exogenous hormone Put in the process of somatic embryogensis.when add the inhibitor the Torpedo-shaped embryo times SOD activity is high,the SOD activeness is low with mature of the embryo.The POD activeness maintains at the rise when the process of similarly inhibitor.3. It is different that the endogene PA content of the somatic embryogenesis and the mature each stage.(Globular embryo,Torpedo-shaped embryo,Heart-shaped embryoid and Cotyledon embryo) 30mg/L exogenous hormone Put may enhance endogene PA's content.The endogene PA's content increases obviously in the sphere embryogenesis times when add exogenous hormone Put.The somatic embryogensis mature period endogene PA's content drops. It is need that maintenance high level's Put/Spd and Put/Spm are clavate pre-embryo growth,does not favor to the somatic cell embryo to be mature.4. 2 inhibitor (CHA and MGBG) process analysis result basic similar. When independent increase inhibitor (CHA or MGBG), only has the inhibitory action to endogene Spd, increases endogene Spm and the Put content; When with the Put simultaneous working, reduces endogene Spm and the Put content obviously. Thus the judgment inhibitor (CHA or MGBG) and the Put simultaneous working is only then plays the inhibitory action to endogene Spm and the Put content. The independent inhibitor (CHA or MGBG) process, through suppresses endogene Spd to reduce the total multi-amine content.