Effects Of Ca Stress On The Activity Of PE, PG, Cellulase, Amylase And Spongy Tissue Disorder In Zihua Mango Fruit (Mangifera Indica L.)

Zihua mango(Mangifera indica L.)was used as materials,with the treatments of EGTA and lime,for studying the effects of Ca stress on the activity of PE,PG, cellulase,amylase and spongy tissue disorder in Zihua Mango fruit,as well as the occurring rule and grade of spongy tissue disorder.It is attempted that the relationship between activity of PE,PG,cellulase and amylase and spongy tissue disorder in the fruit growing process is faund through studying the effects of Ca stress on the activity of these enzymes,for providing the reference to control this disease in the production.The results were showed as follows:1.Spongy tissue disorder was occurred at 10 days before harvest.The higher maturity the fruit was,the severer the disease occurred.The activity of PE,PG and amylase in diseased fruit was higher than the control,especially,the more evidently in the late stage of fruit maturity.It is showed that has close relationship between the activity of PE,PG and amylase and the occurring of spongy tissue disease in the late stage of fruit growing.2.The disease rate and the disease index of spongy tissue were higher with the increasing of fruit weight,and the activity of PE,PG,cellulase and amylase in the big fruit was higher than the small fruit.3.The shortage of calcium in Zihua mango fruit was induced with the treatment of EGTA,and increased the rate of spongy tissue disease.At the same time,the activity of PE,PG and cellulase was increasing with the short of calcium,and the values of Ca/P,Ca/Mg and Ca/K were decreased.4.The Ca content in Zihua mango fruit was increased with the treatment of lime,and increased the values of Ca/P,Ca/Mg and Ca/K,and controlled the activity of PE,PG,cellulase and amylase.To a certain extent,the physiological disorder of spongy tissue in Zihua mango fruit was decreased with the treatment of lime5.The activity of PE,PG,cellulase and amylase in the fruit of different diseased grade was higher than the control.In addition,Comparison with the control,the change values of cellulase activity of diseased fruit in storage were more evidently than in the growing time,but the change values of amylase activity were quite the contrary with it.The results showed that it had a certainly order on the effects of these enzymes on the spongy tissue disorder.