Study On Differential Proteomics And Physiology Change Of Mycelium Of Hypsizigus Marmoreus In Cold Stress

Hypsizigus marmoreus is a kind of edible fungi which is valuable in edible and medical. It plays an important role in domestic market and foreign trade. It is need to be stimulated in low temperature while it begins fruiting. Temperature is the main factor that effects the growth and quality. In this research, we used proteomic methods and techniques, analyzed the variation of proteome in mycelium with suitable and low temperature. In order to search the different proteins related closely to cold stress and to investigat the function. Meanwhile we analyzed the activities of protective enzymes under two different conditions to know the adaptive mechanism of edible fungi in cold stress, as the basis of clone the genes inducted by low temperature in the future.This study use the optimize tempertature (25℃) as control, 14℃as treatment. By using two-dimensional electrophoresis (2DE) and mass Spectrometry (MS) techniques, we analyzed the proteins between CK and treatment in different treating time. Twenty-six protein spots were found changed under cold stress conditions. Changed protein spots were identified first by MALDI-TOF-MS/MS, there were fourteen proteins were successfully identified.In the fourteen differental proteins, actin proteins and tublin proteins are the important components of cytoskeleton; Proteasome and acetolactate synthase are involved in the synthesis and decomposition of protein; ATP synthase, transaldolase, formate dehydrogenase, and RuBisCO are concerned with the metabolize of energy and substance; guanine nucleotide binding protein and 14-3-3protein participate in control of signal transcription; nascent polypeptide-associated complex may be related to the differentiation and development of cell. It shows that there may be kinds of proteins and enzymes joined in the control of Hypsizygus marmoreus in low temperature.We also studied the activity of protective enzymes. The activity of CAT,POD,SOD raised rapidly in the pre-9 hours of treatment. And were higher than those in control. After that they dropped gradually. MDA increased in the early 12 hour, later slightly declined. It accounted for the membrane of hypsizigus marmoreus were injured. But the hypsizigus marmoreus possess strong adaptability in a range of low temperature.