The Optimization For The Liquid Fermentation Technics Of Hypsizigus Marmoreus And Analysis Of The Fermengtation Production

Hypsizigus marmoreus is one of the nutritious rare fungus for edible and officinal use. It's a kind of potential producing breed. However, the traditional planting method of Hypsizigus marmoreus, that is solid spawn planting method, is of long cycle, low efficiency, and low commercial value, therefore it restricts the industrialization and scale of Hypsizigus marmoreus. So the research on technics and application of Hypsizigus marmoreus liquid spawn was carried through. We used the orthogonal test to optimize the liquid fermentation technics of Hypsizigus marmoreus. Then we compared it to the traditional solid spawn planting method of Hypsizigus marmoreus. And we used the chemistry and GC-MS chemical fingerprint collection of illustrative plates method to analyse the chemical components of the ferment production. This research afforded the theory foundation and application value for the industrialization of Hypsizigus marmoreus and for the further exploitation of Hypsizigus marmoreus functionality food. The main research output is following:1,The effects of some nutritional factors on the submerged fermentation of Hypsizigus marmoreus were studied in this article. An optimum medium was obtained through single factor tests and optimization tests. And the study on submerged culture of Hypsizigus marmoreus 9603 determined the optimum carbon source and the nitrogen sources. The result showed that potato, glucose, yeast powder were favorable to the mycelium growing of Hypsizigus marmoreus. An optimum medium was obtained by the further orthogonal test. The optimum medium composition of fermentation was as follows: potato 20%, glucose 3%, yeast powder 0.2%,KH₂PO₄ 0.1%, MgS0₄·7H₂O0.1%.2,The submerged culture conditions for Hypsizigus marmoreus 9603 were studied with the method of shake flask. The result showed that the optimum submerged culture conditions for Hypsizigus marmoreus 9603 were listed as follows: the stirring speed at 120 r/min, temperature at 20℃, initial pH at 6.0-7.5(not necessary to regulate), inoculation volume at about 5ml(250mL erlenmeyer flask, aeration ratio at 100ml), inoculation quantity 5%.The aeration ratio at 100mL(250mL erlenmeyer flask). 3,During the culture process of Hypsizigus marmoreus 9603 liquid spawns, the variation of mycelial pellet numbers, pH value, the contents of reducing sugar and amino nitrogen and the enzyme activity were studied. The results showed that the numbers and activity of mycelial pellets were related to those indexes, and that the quality of the liquid spawn was the best when the contents of reducing sugar and amino nitrogen were 0.98g/100mL and 7.52mg/100mL resp, and the activities of cellulase and Proteinase were 7.85U and 12.6U resp. The number(sixty per milliliter) and activity of pellets reached largest when the pH value reached 5.20, and the fermentative period on the 7th day.4,The experiments to compare liquid and solid spawn of Hypsizigus marmoreus and the benefit analysis were carried out. The results showed that contrast to solid spawn culture, the liquid spawn had a lot of advantages such as short cycle, orderly growth and high efficiency. The promising prospect of liquid spawn was proved adequately. The inoculum of 15 mL per hole is the best. During the range of 0-25℃, the viability of liquid spawn of Hypsizigus marmoreus 9603 was minus relative to the temperature and time of storage. The optimal temperature is 0-10℃, The optimal time is 3-9 days. The mycelia of liquid spawn by centesis grew 2.21 mm per day. The mycelia of liquid spawn by surface grew 1.71 mm per day , and the mycelia of solid spawn grew 1.21 mm per day. The biology efficiency of Hypsizigus marmoreus 9603 which was cultivated on liquid spawn by centesis was accounted 64.80% for the dry weight of medium, the corresponding yield of liquid spawn by surface and solid spawn were 63.40% and 51.00%. Contrast to solid spawn culture mode, the fugus growed more orderly by the liquid spawn mode while the other characters were the same.5,The chemical components of the Hypsizigus marmoreus fermentation solution were analyzed. Systematic pre-experiment showed that the fermentation solution of Hypsizigus marmoreus 9603 contained amino acid, protein, polypeptide, organic acid, saponin, inner ester, coumarin, naphtha, hydroxybenzene, sterol and triterpene. Systematic pre-experiment also showed that the fermentation solution of Hypsizigus marmoreus 9603 didn't contain alkaloid, tan and cyanogen. By method of GC/MS, the chemical components of the volatile gas in fermental solution of Hypsizigus marmoreus 9603 contained alkane, mellow, ketone, aldehyde and other kinds of substance. And mellow (7.46%) and ketone (7.13%) is in the majority. The chemical components in the chloroform extraction of the Hypsizigus marmoreus 9603 fermental solution were determined, too. The result showed that it contained alkane, hydroxybenzene, ester, mellow, ketone and other kinds of substance. And alkane (12.76%) and hydroxybenzene (10.63%) is in the majority. Among these substances, plenty of them have been proved to possess good health care function such as anti-oxidation or anti-caducity function. This research afforded a certain theory foundation and application value for the further exploitation of Hypsizigus marmoreus functionality food.