Effect Of Hydrogen-rich Water On Yield And Quality Of Basidiomycetes Hypsizigus Marmoreus In The Industrialized Production

Hypsizygus marmoreus is one of the main edible fungi in the industrialized production.Due to the requirement of a long spawan-running process for H.marmoreus,the instability phenomenon has occurred in the industrialized production process.In the previous study,we found that adding Hydrogen-rich water(HRW)after scratching mycelia could make the production stability on a certain extent.Furthermore,we also study the effects HRW on the yield,quality,preservation of the fruiting body after postharvest and growth of H.marmoreus.And we also explored the possible mechanism of HRW on yield of H.marmoreus.These research results are as follows:(1)We selected high yield strain of SIEF3154 and low yield SIEF3153 strains of H.marmoreus as the research object.The study found that HRW could effectively improve 7%-23%yield of the two kind strains of H.marmoreus and stable the yield of H.marmoreus at a high level.Among them,the production of low yield SIEF3153 strains was increased higher than the high yield strain SIEF3154.Further studies showed that polysaccharide and total sugar of H.marmoreus treated with 0.450mmol/L HRW were increased by 34.5%and 15.5%compared with the control group,and the total amino acid content also was increased by 12.2%.Therefore,the 0.450mmol/L HRW treatment could improve the quality and flavor of H.marmoreus matter accumulation.Compared with the control,0.225mmol/L HRW treatment could delay the decrease of fruitbody hardness,anti-oxidase activity and soluble protein after storage sixth days of H.marmoreus.0.225mmol/L HRW treatment could also slow the increasing rate of weight loss,relative conductivity,malondialdehyde(MDA)and the content of superoxide anion,thereby prolonging the shelf life of H.marmoreus.(2)Different concentrations of HRW treatment compared with the control,the key developmental stages of antioxidant enzymes activity in the growth of H.marmoreus(mycelial regeneration and Mycelial pigmentation stages)were higher than control;But in the Primordium,Naihead and Flat ball stages,the activities of antioxidant enzymes were lower than those of the control,and the difference between the 0.900mmol/L HRW treatment and the control was the most significant;The transcriptional level results were in accordant with these results.(3)The results showed that Ca2+signaling pathway,MAPK signaling pathway,ROS signaling pathway and Oxylipins signaling pathway were up-regulated in the treatment of HRW,Some genes involved in these pathways could be up to more than ten times.The industrialized production strain SIEF3133 was used to shake flask test;On the simulation of scratching conditions of H.marmoreus,we found that HRW treatment could increase the activity of enzyme in the damaged mycelium of glutathione system enzymes and catalase,But superoxide dismutase(SOD)had no significant difference after HRW treatment.The HRW treatment could reduce the MDA in the injured mycelium.After 9,11,13 days culture,it was found that the HRW treatment could increase the activity of Laccase and manganese peroxidase,and the biomass of the injured hyphae;However,the relative expression levels of the Laccase and manganese peroxidase at the transcriptional level were not as same as that of the enzyme activity.In summary,this study demonstrated that HRW could improve and stabilize the effective yield of H.marmoreus,appropriate concentration of HRW can improve the quality of H.marmoreus,prolong the shelf life of H.marmoreus,These study has potential economic value;To reveal the HRW through the influence of H.marmoreus related enzymes,thereby affecting the growth of H.marmoreus.Further,in order to elucidate the mechanism of HRW could yield stability of H.marmoreus at a high level,has made some useful exploration,and has certain theoretical significance.