Assessment Of AFLP And ISSR-based Genetic Variations Of Provenances And Cultivars Of Phyllostachys Edulis

To provide molecular biology theories for the selection of good provenances and hybrid breeding of Phyllostachys. edulis, Amplified fragment length polymorphism (AFLP) and Inter-simple sequence repeat (ISSR) markers were employed to analyze genetic variations of 17 provenances of P. edulis. AT the same time, to provide references for the bamboo systemamics of intraspecies and interspecies of P. edulis, genetic similarity as well as relationship among 10 cultivarls of P. edulis and 2 close species were evaluated by the same means.Main results obtained as follows:(1) Statistical analysis by Mantel test showed that genetic distance matrices from AFLP and ISSR data were significantly correlated (r=0.925, P=0.005 for the study of 17 provinances of P. edulis; r=0.959, P=0.013 for the study of 10 cultivars of P. edulis). In addition, both AFLP and ISSR were able to distinguish17 provenances , 10 cultivars of P. edulis and 2 close species. Our data suggested that AFLP and ISSR techniques as well as data created with these techniques were highly reliable. Therefore, AFLP and ISSR techniques were very useful tools on the study of the intraspecies and interspecies of a bamboo species.(2) Results based on the study of 17 provinances of P. edulis: total 523 bands, in which 199 are polymorphic,were exhibited using 12 pairs of AFLP primers whereas total 138 bands, in which 55 bands are polymorphic, were exhibited using 16 ISSR primers. 17 provenances of P. edulis were divided into 6 groups, but 4 previous good provenances of P. edulis belonged to different groups based on the UPGMA clustering. 17 provenances of P. edulis shared a high genetic similarity. Neverthless, some genetic variations were found and genetic distance among them ranged only from 0.020 to 0.182 and the average value was 0.067.(3) Results based on the study of 10 cultivars of P. edulis: total 827 bands, in which 495 are polymorphic, were exhibited using 15 pairs of AFLP primers whereas total 231 bands, in which 154 bands arepolymorphic, were exhibited using 16 ISSR primers. The dendrogram generated with AFLP and ISSR markers indicated that 10 cultivars of P. edulis had a high similarity with genetic distances ranging from 0.023 to 0.108 and the average value was 0.078, and could be divided into three groups based on their genetic variations. Meanwhile, our study supported that P. edulis cv. Gracilis and P. edulis cv. Heterocycla were two cultivars based on genetic distances from two molecular markers.