| Four experiments were conducted to research the effect of addition of different fats in dietary on rumen fermentation of sheep in vitro and fatty acid profiles in milk fat from lactating ewe.Experiment 1:The objective of this study was to investigate the effect of addition of different fats in dietary on rumen fermentation of sheep in vitro.The addition levels of fats were 0.0% (control group),1%,2%and 3% respectively.The results showed:â‘ Different levelss of cottonseed oil de- creased the gas production(GP)of rumen fermentation in vitro,but the team was not significant (P>0.05).Compared the control group, cottonseed oil did not affected significantly pH and ammonia nitrogen(NH3-N).Compared the control group, cottonseed oil can decreased carboxymethyl cellulase (CMCase) and xylanase(XYLanase) activity,CMCase of the control group were significant(P<0.05), but the treatment was not significant (P>0.05) at 48h.Compared the control treatment,with increas- ing the addition levels of cottonseed oil,that decreased degradation rate of dry matter (DM),neutral detergent fiber (NDF) and Acid Detergent Fiber(ADF).At 48h,to DM,compared the control group, 2% and 3%group were low(P<0.05), but the treatment was not significant (P>0.05).At 72h,to ADF, compared the control group,2% group were significantly (P<0.01),3% group were significantly (P<0.05).Compared the control group, cottonseed oil did not influence general volatile fatty acid concentration(TVFA),the mole percentage of acetate and propionate, the ratio of acetate to propionate(P>0.05).â‘¡Different levelss of sunflower oil decreased GP of rumen fermentation in vitro,compared the control group,3% and 2% group were significantly (P<0.05), but between 2% and 3% was not significant (P>0.05).Compared the control group, sunflower oil did not affected significantly pH and NH3-N in rumen.Compared the control group, sunflower oil can decreased CMCase and XYLanase activity,at 24h, 3% and 2%group were significantly(P<0.05), but between 2% and 3% was not significant (P>0.05).Compared the control treatment,with increasing the addition levels of sunflower oil,that decreased degradation rate DM,NDF and ADF (P>0.05).Compared the control group, sunflower oil did not influenceTVFA,the mole percentage of acetate,propionate and butyrate,the ratio of acetate to propionate (P>0.05).â‘¢Compared the control group ,different levelss of safflower oil decreased GP of rumen fermentation in vitro. but among the team was not significant (P>0.05).Compared the control group, safflower oil did not affected significantly pH,NH3-N,CMCase and XYLanase activity in rumen. With increasing the addition levels of safflower oil,that decreased CMCase and XYLanase activity. Compared the control treatment,with increasing the addition levels of safflower oil,that decreased degradation rate DM,NDF and ADF. At 72h, compared the control group,NDF of the treaement group were significantly(P<0.05), but its was not significant (P>0.05). At 24h, compared the control treatment,ADF of the treaement group were significantly(P<0.05), but among its was not significant (P>0.05). Compared the control group, safflower oil did not influenceTVFA,the mole percentage of acetate,propionate and butyrate,the ratio of acetate to propionate(P>0.05).â‘£Different levelss of fish oil decreased GP of rumen ferme- ntation in vitro(P<0.05).Compared the control group,fish oil of 2% and 3% heightened significantly pH (P<0.05) at 72h . Compared the control group, fish oil did not affected NH3-N. Compared the control group,fish oil of 2% and 3% group decreased significantly CMCase(P<0.05) at 24h. Compared the control group,fish oil of 2% and 3% group decreased significantly CMCase(P<0.01) at 48h. Compared the control group,fish oil of 3% group decreased significantly XYLanase(P<0.05) at 48h.Compared the control treatment,with increasing the addition levels of fish oil,that decreased degradation rate DM,NDF and ADF (P<0.05). Compared the control group, fish oil did not influence TVFA,the mole percenTGe of propionate and butyrate,the ratio of acetate to propionate(P>0.05). Compared the control group,fish oil of 2% and 3% group decreased significantly the mole percentage of acetate (P<0.05).To the ratio of acetate to propionate, 2% group was significantly (P<0.05),3% group was significantly (P<0.01).These results indicated that the addition levels of vegetable oil is3%of DM, the addition levels of fish oil is1%of DM.Experiment 2:The objective of this study was to investigate the effect of 4 different fats(cotton oil,sunflower oil,safflower oil and fish oil)of sheep dietary on rumen fat hydrolysis and hydrogenation by gas production in vitro,thin-layer chromatography(TLC) and gas chromatographic technique. Experiment used one way trial design the addition levels of vegetable oil is3%of DM, the addition levels of fish oil is1%of DM. The results showed:â‘ Adding fats in diet can raise significantly fat hydrolysis rate (P<0.05), hydrolysis rate of the control group,cotton oil,sunflower oil,safflower oil and fish oil were 63.84%,74.82%,81.89%,83.02% and 72.49% respectively.â‘¡Adding fats in diet can increase significantly proportion of C16:0,C18:0,C18:1 and C18:2 (P<0.05). Beside cotton oil, others can decrease significantly SFA/UFA(P<0.05). But among the three groups was not significant,the lowest proportion is safflower group.Experiment 3:The objective of this study was to investigate the effect of different fats combinations of sheep dietary on rumen fat hydrolysis and Hydrogenation by gas production in vitro,TLC and gas chromatographic technique. Experiment used one way trial design, the overall addition levels of fats is 3%of DM, fish oil is 1%of DM.The results showed:â‘ Adding fats combination in diet can raise significantly fat hydrolysis rate (P<0.05). Of which, hydrolysis rate of the combination of the sunflower oil and fish oil is up to 79.88 percent, but between it and safflower oil group was not significantly different(P>0.05),and between it and cotton oil was signi- ficantly(P<0.05).â‘¡Adding fats combination in diet can increase significantly proportion of C16:0,C18:0,C18:1 and C18:2 in rumen (P<0.05). In which,the rate of increase of C18:1 was most obvious in all acids. Beside cotton oil, others can decrease significantly SFA/UFA(P<0.01). But among the two groups was not significant,the lowest proportion is safflower group.Experiment 4:The objective of this study was to investigate the effect of different fats combinations of sheep dietary on composition of sheep milk fatty acids.Four sheeps of same number of young stock and approximately physical condition were used in one way trial design.All sheep were offered alfalfa hay,cottonseed,corn silage and concentrate feed. The rate of concentrate and crude is 50:50.The overall addition levels of fats was 3%of DM, in wich fish oil is 1%of DM.Each experimental period is 8 days,the last 3 day was the period of adaptation to the diet.The results showed:in addition to C18:0,content of C8:0,C10:0,C12:0,C14:0 and C16:0 in the milk fat were decreased by adding fats combination in diet.Of which, compared the control group,content of C8:0,C10:0,and C16:0 of the treatment group were significantly(P<0.05).And content of unsaturated fatty acid in the milk fat were raised by adding fats combination in diet.compared the control group,content of C16:1of the treatment group were significantly(P<0.05).In addition to combination group of the sunflower oil and fish oil,content of C18:1of others were significantly(P<0.05). In addition to combination group of cotton oil and fish oil,content of C18:2of others were significantly(P<0.05).In which, safflower oil group is best.Through the above analysis we can see that Effect of adding combination of different fats in diet to the fatty acid composition of milk fat is good.The improvement is conducive tothe development of human health.In wich , combination of sunflower oil and fish oil and combination of safflower oil and fish oil were relatively good results. |
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