| With the improvement of people’s living standard, animal foods take up more and more proportion in daily diet structure, which could be a cause of some diseases such as cancer, cerebral thrombosis, obesity, diabetes and so on. People could have some diseases if absorbing more unsaturated fatty acid, so people pay more attention to the relationship of health and diet. CLA is the unique animal resource functional fatty acid, which was recognized internationally. In order to increase the contents of CLA in animal food, we could feed animal vegetable oil to meet animal’s requirement for unsaturated fatty acid and raise the contents of CLA in animal. Likewise, with the progress of the society and development of the economy, the effect of global warming continues intensifying. About14%greenhouse gases in the world are produced by agriculture. Methane is the rumen digestive products of ruminant animals.About2%to5%of the total feed energy which ruminant animals intake are losing in the form of methane releasing to the air. Releasing methane not only has the negative effects on animals production efficiency, but also increases the greenhouse effect. It is obvious that regulating methane release of ruminant animals has very important research values in protecting natural environment and enhancing animal’s production efficiency.This paper mainly studied the effects of linseed oil and malic acid adding into the diet on rumen fermentation and fatty acid composition of rumen fluid. We selected4Yanbian Yellow Cattle installing a permanent rumen fistula and used4×4Latin square design. In this test, there was a control group and three trial groups. Feed the control group the basal diet and add Linseed oil475g/d (the LO group), Linseed oil475g/d+Malic acid160g/d (the LOM group) and Linseed oil475g/d+Malic acid160g/d+baking soda (NaHCO3)47.5g/d (LOMS group) into the trial groups on the basis of the basal diet. The results were as follows, the pH value of each had increasing tendency compared with the control group, trial groups after feeding9h increased significantly (P<0.05), LOMS group at6h was significantly higher than the control group(P<0.05).Compared with the control group, the propionic acid concentration of each trial group had increasing tendency, while the acetic acid, butanoic acid and total volatile fatty acids had reducing tendency. And groups added Linseed oil and Malic acid had more significant effect. Compared with the control group, the ratio of acetic acid and propionic acid in the treatment group reduced significantly (P<0.05). Adding Linseed oil increased the content of fatty acids in rumen fluid. Diet adding Linseed oil (LO group) raised the content of C16:0, C18:0, C9-C18:1, t11-C18:1, c9, t11-CLA, t10, c12-CLA and C18:3remarkably in rumen fluid. And there were no significant differences at any time point (P<0.05). It had significant differences in the content of t11-C18:1between LOM group and LOMS group at1h and9h (P<0.05).Adding linseed oil into the diet could reduce the degradation rate of DM, OM, CP and NDF of rumen. At the same time, adding linseed oil and malic acid could improve the degradation rate in certain extent. The results showed that adding linseed oil and malic acid into diet was beneficial to rumen fermentation of Yanbian Yellow Cattle and the effect of adding both of them at the same time was better, adding linseed oil could raise the content of unsaturated fatty acid and CLA in rumen fluid. |
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