Effects Of Perilla Oil And Rubber Seed Oil Supplements On Rumen Fermentation Parameters, Fatty Acid Composition And Methane Production By A Rumen-simulation Technique

A completely random experiment desigen was carried out to investigate theeffect of plant essential oil with different dose levels on rumen microbial fermentationby Rumen-Simulation Technique in vitro. The objective was to select suitable plantoil type and dose which promote rumen fermentation and inhabit methane production.The five supplemental levels (0%,1%,2%,3%,4%of DM) of perilla oil and rubberseed oil were used as the treatment factors, there were three replicates per treatmentand each experiment lasted for eight days.Experiment1. The results indicated that different doses of perilla oil had nosignificant effect on the pH and ammonia (NH3-N) content of fermentation broth (P>0.05). Compared with control, the degradation rate of dry matter, neutral detergentfiber, acid detergent fiber and gas production of fermentable substrates significantlydecreased with perilla oil dose increase(P＜0.05). Meanwhile, the content ofpropionic acid, acetic acid and acetate propionate proportion significantly decreased(P＜0.05) while the effect of perilla oil on TVFA was insignificant (P＞0.05). Methaneproduction treated by different doses of perilla oil has a downside (P＞0.05) while theconcentration of C18:1, c9, t11-CLA and total long chain fatty acids significantlyincreased (P＜0.01).Besides, the relative expression of cellulose degradationbacterium (R.albus, B.fibrisolven) and methanogen decreased significantly. In aconclusion，adding an appropriate dose of perilla soil in diet could change rumenfermentation type, reduce methane production and regulate the fatty acid compositionof rumen fluid.Experiment2. The results indicated that different doses of rubber seed oil had nosignificant effect on the pH and ammonia (NH3-N) content of fermentation broth(P>0.05). Compared with control, the degradation rate of dry matter, neutral detergentfiber, acid detergent fiber and gas production of fermentable substrates significantlydecreased with rubber seed dose increase(P＜0.05). Meanwhile, the content of aceticacid and acetate propionate proportion significantly decreased (P＜0.05) while the effect of rubber seed oil on TVFA was insignificant (P＞0.05). Methane productiontreated by different doses of rubber seed oil significantly decreased(P＜0.05) whilethe concentration of C16:1, C16:0, t11-C18:1, t9-C18:1, c9, t11-CLA and total longchain fatty acids significantly increased (P＜0.01). Compared to control (0%DM),the relative expression of B.fibrisolven and F.succinogene treated by4%DMsignificantly decreased(P＜0.01) while R.albus and methanogen treated by3%DMsignificantly decreased(P＜0.01).In a conclusion，adding an appropriate dose ofrubber seed oil in diet could change rumen fermentation type, reduce methaneproduction and regulate the fatty acid composition of rumen fluid.