| At first, a detailed literature review of the biology on sea cucumber, Apostichopus japonicus and the research progress about the effect on some environmental factors. And then, a series of indoor experiments are conducted to investigate the effects of light color on the behavior, growth, energy budget and metabolism with three specifications sea cucumber, Apostichopus japonicus. The results are summarized as follows:2.1 The effect of light color on behavior of sea cucumber, Apostichopus japonicusThe experiment is carried out with a diameter of 2m circular tank, with the temperature controlled in 18℃. Experimental Observation of a six day cycle, are set up Red, Orange, Yellow, Green, Blue and White control group, six kinds of light treatments, a total of five repeat. Experimental results show that sea cucumber, within 24h, the six-color distribution of sea cucumbers has no significant difference in the dark state, but during photophase, the distribution of sea cucumbers has a significant difference under different light color; the order of the photokinesis under different light color is Yellow> Orange> Red> White> Green> Blue, the photokinesis in Yellow and Orange is significantly higher than that of Green and Blue (P <0.05), and Red and White have no significantly difference compared with Orange and Green(P>0.05); In addition, the everyday photokinesis has no significant change during an experiment cycle. The results show that there is a significant effect of photokinesis under different light color, and the sensitive area is between orange and yellow.2.2 The effect of light color on the growth of sea cucumber, Apostichopus japonicusThis experiment used indoor controlled study of the experimental methods under different light color on the growth of sea cucumber, Apostichopus japonicus. Experimental sustained 60 days. Experiment sets up the Red, Orange, Yellow, Green, Blue and White control group, six kinds of light treatment. The results show that the growth of sea cucumber has a significant difference under different light color, which the growth of Red light is significantly higher than the growth of Blue light (P <0.05), the other four treatment with the growth of color in between ; the treatment groups'feeding rate (FI) have no significant difference (P>0.05), but dealt with in the Blue food conversion is efficiency (FCE) far below the Red and Orange-treated group, as well as White and Yellow-treated group, and Green deal with the transformation efficiency has not significant difference; in Blue light feces production rate (FPR) is significantly higher than that in Red and Yellow light, and the other lights have no significant difference from the point of view of energy, Blue light for Growth (G) is the minimum energy, significantly lower than Red, Yellow and Orange three kinds of color, with White and Green treatments with the significant differences. The experiment results show that Blue light group is in the slow growth, significantly lower than the growth of Red light, but with the control group has no significant difference. This is because that the Blue light group food conversion efficiency (FCE) is minimum, for the growth of its energy (G) minimum, which eventually led to Apostichopus japonicus in the Blue light of slow growth.2.3 The Effect of Light Color on Metabolizability of Sea Cucumber, Apostichopus japonicusThis study used mainly controlled indoor studied under different light color specifications Apostichopus japonicus three kinds of oxygen consumption rate and ammonia excretion rate changes. Experimental design of the Red, Orange, Yellow, Green, Blue and White control group, six kinds of illumination, as well as big [(73.32±1.73) g], [(46.71±0.15) g], small [(25.67±0.25) g] three kinds of specifications. The results show that: (1)The color on the rate of oxygen consumption and ammonia excretion rate has significant influence. The impact on the three kinds of specifications has the same trend as the rate of oxygen consumption, White> Red> Yellow> Green> Blue> Orange, three kinds of specifications has the same change trends as ammonia excretion rate, White> Red> Orange> Green> Yellow> Blue ;(2) The regressive equation between the body weight of sea cucumber and oxygen consumption can be described as R = aWb, the ranges of a and b are 0.0059-0.0121, with a mean of 0.0101and 1.1197-1.2146, with a mean of 1.1583 ;(3) The regressive equation between the body weight of sea cucumber and ammonia excretion rate can be described as N = aWb, the range of a and b can be expressed as 0.0042-0.02, with a mean of 0.0086 and 0.4553-0.8678, with a mean of 0.6665. On the condition of the experiment the rate of oxygen consumption depresses with light color shorter, and the Ammonia rates have the same trends.
|
PDF Full Text Request