| Two types of explants from Abelia grandiflora'Francis Mason'including young stems and leaves, were used to study the organogenesis, including the direct organogenesis and indirect organogenesis in this experiment.1. Direct organogenesis of Abelia grandiflora'Francis Mason'Focus on the sterilization of explants, the best medium fomula and culture procedures. The contents and test results were as follows:(1)The stem segments could be sterilized in 15min with 10% 84 solution.The empoison rate of stems was 10%. And the survival rate of stem was 90%; the leave segments could be sterilized in 10min with 10%84 solution. The empoison rate of leaves was 3.3%.And the survival rate of leaves was 96.7%.(2) The best drawing materials seasons were April. The empoison rate was low, and the survival rate was high.(3) The best initination medium was MS+2.0mg/L6-BA+0.05mg/LNAA.The inducement rate could be up to 85%,and the number of axillary buds was 1.7.(4)The best multiplied medium was MS+2.0mg/L6-BA+0.05mg/LNAA +0.5mg/LKT, and the multiplication rate was 4.90. The succeed era period was 35d, and the succeed era algebra was three era.(5)The best rooting medium was 1/2MS+0.5mg/LIBA + 20g/Lsucrose,the rooting rate was 65%.And the root was strong.(6)For nursling of plantlets,the medium with 1 ruby mica and 1 peat.The survival rate of plantlets was 80%.2. Indirect organogenesis of Abelia grandiflora'Francis Mason'(1)The most suitable medium of callus from leaves was MS+0.1mg/L6-BA+ 1.0mg/L2,4-D+30g/Lsucrose+CH0.2mg/L on which well-grown callus could be induced in great number in 30 days.(2)The succeed and preservation medium was 0.1mg/L6-BA+0.5mg/L2,4-D +30g/Lsucrose+CH0.2mg/L.The proliferation multipled up to two or three times,and callus was in good condition.
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