Study On Tissue Culture Of Pseudolarix Amabilis

Pseudolarix amabilis is a plant species of monomorphic genera belong to Pinaceae family,which is endemic to China. Pseudolarix kaempferi is a famous ancient relict plant,and has higher application value.In this study, the stems,dormant buds and mature embryos were used as explants and preliminary study was performed on tissue culture techniques. The primary conclusion was as follows:(1)To the stems and dormant buds, the best way of disinfection is 75% alcohol(30s)+2.5% NaClO(15min)+0.1%HgCl2(6min).And to the mature embryo,the best way of disinfection was 75% alcohol(30s)+2.5%NaClO(15min).(2)Taking the stems,dormant buds and mature embryos as explants,to Screen the most suitable tissue culture explant. The results showed that the rate of mature embryo adventitious buds induction was 66.94%, the rate of callus induction was 63.62% and the rate of callus differentiation was 20.95%?So the mature embryo is the best explants.(3)Adopting L9(34)Orthogonal experimental design on adventitious buds induction,the greatest effect was NAA, secondly was medium type,the smallest effect was 6-BA, The optimum medium was 1/2MS+2.0 mg·L-16-BA. After the successful induction of adventitious buds primordia,placing the explants in the medium of 1/2MS+0.1 mg·L-16-BA+0.01 mg·L-1NAA is good for the formation and proliferation of adventitious buds.The average number of buds was 6.8 and the leafy buds grow sturdy.(4) The optimum medium was 1/2MS+0.02 mg·L-1NAA+1.0 g·L-1AC for seedling.The rooting medium was 1/2MS+0.3 mg·L-1IBA+0.05 mg·L-1NAA,and the rooting rate was 12.43%.But the roots are difficult to continue to grow and gradually death.(5)In the process of mature embryo callus induction, NAA was more suitable than the 2,4-D. Different concentrations of 6-BA and NAA have a significant influence on the callus induction. The optimum medium was 1/2MS+2.0 mg·L-1NAA+1.0 mg·L-16-BA,and the rate of callus induction was 92.28%.(6) In the process of callus proliferation,the more appropriate medium was 1/2MS+0.5 mg·L-16-BA+1.0 mg·L-1NAA. Cultured in the darkness can prevent callus browning.(7) The optimum medium was 1/2MS+1.0 mg.L-1 6-BA+0.3 mg.L-1IBA for Callus differentiation.And the rate of differentiation is 31.09%.