Identify And Estimate The Germplasm Resource Of Rhizomania Resistance Sugar Beet

Rhizomania is one of destructive disease to sugar beet. Breeding varieties with rhizomania resistance is the most effective method to resolve the problem, and the new varieties breeding is based on idioplasm resource with excellent character, therefore, it is very important to identify and evaluate idioplasm resource materials.This study took idioplasm resource materials of Sugar beet with rhizomania resistance as research subject, and identified and evaluated them in morphological, physiological and molecular mark,the main results include:1. There was large difference in 14 morphological indexes among these 31 materials. The correlation had been found out in correlation analysis. According to principal component analysis, the first principal component represented 34% of all information, and plant height, root yield, sugar yield and disease index were the main effect factors. On the basis of cluster analysis, they had been divided into five parts.2. This study identified and evaluated the germplasm resource materials with rhizomania resistance by EST and POD isozyme, the number of electrophoresis zymase band and marked sites were a little in these two isozyme.3. Salting protein of sugarbeet seed had rich bands, and numbers of bands, site distribution and strength of bands all existed obvious difference among the materials, according to SDS-PAGE electrophoresis under optimum gel electrophoresis. On the cluster analysis of bands, tested materials could be divided into five parts.4. ISSR augmentation reaction system and reaction procedure had been optimized. The results showed that the optimized ISSR augmentation reaction system made up of MgCI2 2.5mmol/L, dNTPs 0.25mmol/L, DNA polymerase 1.5UTaq, primer 0.5μmo1/L, Buffer 2μL 10×PCR, DNA stencil 150ng. The optimized condition was 5min pre-denaturization at 94℃, then 30s denaturization 94℃, 30s annealing at 45℃, 1min extension at 45℃, after 35 cycles, 5min extension at 72℃.5. 13 bands had been checked out from the tested materials by optimized ISSR-PCR augmentation system, augmentation procedure and two filtered polymerase, and total bands of augmentation and difference bands existed large change. Tested materials could be divided into six parts according to cluster analysis. 6. On the basis of four identified technique, the results showed that Y07,Y08,Y11,Y13,Y18 may have different gene with the others and unique genotype, so they had been divide into one group in the cluster analysis.