Antiserum Preparation Of Zucchini Yellow Mosaic Virus Luohanguo Isolate (ZYMV-LHG) And Its Nature Host Detection

Siraitia grosvenori(Luohanguo)belongs to Cucurbitaceae, a perennial liana especially grown in Guangxi province, which had important medical and healthcare value. However, Siraitia grosvenori viral disease has been a major problem in its production industry. In this paper, ZYMV-LHG separation, propagation, purification, characteristics in vitro and antiserum preparation were conducted, and the nature hosts in the field were detected using ZYMV-LHG antiserum. The results showed that ZYMV-LHG could be separated by Cucurbita pepo from diseased leaves of Siraitia grosvenori. Symptoms of Cucurbita pepo infected by ZYMV-LHG were vein-clearing, etiolation, and strip-like malformation. The thermal inactivation of ZYMV-LHG point was 55°C. The dilution end point was 10"3. The longevity in vitro was 6 days. ZYMV-LHG was purified from diseased leaves of Cucurbita pepo. The antiserum against ZYMV-LHG was prepared by injected BALB/C mice with purified virus particles. The titer of this antiserum was 1:16 000. The crops and weeds in or nearby the Siraitia grosvenori orchard were detected with ZYMV-LHG antiserum. The results showed that 11 families 14 species had positive reaction with ZYMV-LHG antiserum, including Cucurbitaceae (Luffa cylindric, Cucurbita moschata and Lagenaria siceraria), Solanaceae (Capsicum annuum), Leguminosae (Amphicarpaea edgeworthii), Violaceae (Viola verecunda), Verbenaceae (Clerodendrum cyrtophyllum), Commelinaceae (Commelina communis), Compositae (Crassocephalum crepidioides), Polygonaceae (Rumex dentatus), Amaranthaceae (Alternanther sessilis), Plantaginaceae (Plantago asiatica), Oxalidaceae (Oxalis corniculata). The control efficiencies of several virucides to Siraitia grosvenori viral disease were carried out in greenhouse. In the treatment of spraying virucides before inoculating ZYMV-LHG, 1.45%Ribavirin copper. Triacontanol. Zinc had the highest control efficiency (58.99%). With lower control efficiency (27.19%) was 5% Octyl glycine ethyl amine salt. In the treatment of spraying virucides after inoculating ZYMV-LHG, three virucides had higher control efficiency. There were 52.82% (1.45% Ribavirin copper·Triacontanol·Zinc),50.23% (5% Moroxy dine hydrochioride) and 47.17%(20%Moroxydine hydrochloride·Second copper) respectively. With lower control efficiency (22.07%) was 5%Octyl glycine ethyl amine salt. In brief, the virucides with high efficiency to control Siraitia grosvenori viral disease were not available.