The Detection And Phylogenetic Analysis Of Zucchini Yellow Mosaic Virus From Hunan

Zucchini yellow mosaic virus(ZYMV) belongs to Potyvirus, known as a main pathogen infecting Cucurbits and causing severe yield losses in cucurbits production. Hunan province is also severely afflicted by such virus, but relevant research remain absent in this area, In order to systemically manage ZYMV and alleviate its destruction in agro-economy, our study were mainly conducted in the fallowing aspects:1.Two specific primers named ZYMV-1and ZYMV-2were designed according to the whole genome of ZYMV and successfully used to establish the RT-PCR detect ion method.Combined with RT-PCR, biological methods were applied to detect the zucchini leaf samples with typical symptom of ZYMV infection collected from Chengzh ou,Changsha,Hengyang,Yueyang,Zhangjiajie,Chengde and Loudi in Hunan.Within those samples,54.5%showed infection positive according to the test results,which reinforces the conclusion that ZYMV is the main pathogen damaging cucurbit production in hunan.2.The amplicon ZYMV HC-Pro amplified by primer ZYMV-1was sequenced and its length was621bp.Nib sequence, CP whole sequence and3-UTR sequence were amplified by primer ZYM-l,and shown length as80,837,168bp respectively.Besides, based on the analysis of7CP genes and5HC-Pro genes from our obtained samples, we speculate that those fragments have different origins.3. The recombinant plasmid was constructed based on the core area of highly conservative HC-Pro sequence with length of193bp, and used to establish the qPCR method to detect ZYMV.In the Q-PCR reaction,amplification efficiency was91.9%,with the correlation coefficient of0.998and detection range of9.84×101copies/ μL～9.84x107copies/μL.