Reserach On The Mechanism Of Evaluation Method Of Spawn Quality Of Hypsizygus Marmoreus And Development Of Kit

Hypsizygus marmoreus is one of the main well-known edible mushrooms that has been realized industrial production.Spawn quality is the fundament to the development of edible industry.However,due to the long cultivation period of Hypsizygus marmoreus,the inferior strains could be found when fruiting and it can take huge losses to mushroom industry.So,it is urgent to find a quick and easy evaluation method of spawn quality.Our group had found that the normal and degradation strain of Hypsizygus marmoreus had different decolorization ability to synthetic dyes,the results could be obtained within 14 days and the correlation between showing color and yield could reach 0.880;the correlations between color results of the method and main commercial characters of Hypsizygus marmoreus with the pileus diameter and thickness,stipe length and diameter was confirmed.Thereforce,the present study is focused on further investigating the mechanism of liquid bromothymolblue lactose(LBL)evaluation method of Hypsizygus marmoreus and optimizing the production process of kit which is based on the method.The changes of related physiological indicators during liquid culture of of two Hypsizygus marmoreus strains that were yellow and blue in LBL assay was studied,and the correlations between them was studied;the different of the active substance that decolorized bromothymolblue(BTB)in fermentation broth of pros and cons of strains was compared by separation and analysis means such as dialysis,thinlayerchromatography(TLC)and gaschromatograph-massspectrometer-computer(GC-MS);the color effects between bromothymolblue(BTB)and saponin in fermentation broth of pros and cons of strains was analysised;the process parameters of production of Hypsizygus marmoreus quality detection kit was optimized and established.The resaults could be generally categorized under four headings as following.1.The A615,the pH,the conductivity,the mycelial biomass,the lactose of two Hypsizygus marmoreus strains that were yellow and blue in LBL assay was determined during liquid culture,and the correlations between them was studied,and then the action position of BTB was also studied.The results showed that the changes of pH and conductivity of SIEF3132 that was yellow in LBL assay were lower than that of SIEF3246 that was blue in LBL assay;the ability of metabolizing lactose of SIEF3132 was higher than that of SIEF3246;the active substance that decolorized BTB was secreted into vitro and not found in the vivo.2 The nature of the active substance that reaction with BTB in fermentation broth of Hypsizygus marmoreus was studied.The results showed that the molecular weight of active substance that decolorized BTB was less than 1000.The volatile component have significant differences in fermentation broth of pros and cons of strains:the aldehyde content of SIEF3132(74.138%)was higher than that of SIEF3246(50.352%),and the alcohols(16.680%),furan(8.379%),olefin(6.813%),benzene(6.265%)and esters content of SIEF3246(4.620%)was was higher than that of SIEF3246.3 The saponin in the fermentation broth of Hypsizygus marmoreus SIEF3132 and SIEF3246 strain was extracted with N-butanol extraction method and the content of saponin was compared.The results show that the color of BTB with the saponin that extracted in the fermentation broth of Hypsizygus marmoreus SIEF3132 and SIEF3246 strain was yellow and the saponin content of SIEF3132(1.3767g/ml)was higher than that of SIEF3246(0.9823 g/ml).4 The Hypsizygus marmoreus quality detection kit was developed.The package methods of main compenent of the kit was established:the lactose,peptone,yeast extract were used a liquid package system,the each content in a 1.5ml centrifuge tube was 0.25g,0.375g,0.225g;as the BTB is insoluble in water,so it was dissolved in dilute alkali solution,and the content in a 1.5ml centrifuge tube was 1.25x10-3g.The repeatability and stability of the kit were tested.The results show that the kit was stable within three months at 4 ?.The color card of assay medium and the fitting equation of A615 were made;the package of the kit was designed,and the reagent was distinguished with different color caps.