Orchard Interplanting Different Forage Grass To Soil Microbial Diversity Influences

Microbial organisms play a key role in energy and nutrient cycling, plant litter decomposition, soil productivity maintenance, plant growth and plant disease protection within the agricultural ecosystems. Due to the selectivity of the traditional methods, only 1% of the total population of soil microbial organisms can be cultured and limited data is available concerning how closely they reflect the actual composition of these communities. In this study, molecular methods were used to examine the microbial diversity relationships in the orchard ecosystems. The results are as follows:1. Soil bacterial communities were examined by culture-independent means, namely, 16SrDNA-nested PCR-DGGE profiling. Crude DNA was extracted from soil used to grow different plants, amplified with bacterial specific primers of 16SrDNA by PCR, and separated by denaturing gradient gel electrophoresis (DGGE).Subsequently, bands on DGGE gel were cloned sequenced and the resultant information was used to infer the diversity of bacteria in different soil. The results showed that soil samples colleted from same area had a high identity in DGGE bands patterns. Bacterial community structure was significantly influenced by different seasons. The effects were closely related to the kind of plants. Compared with the control, the grass grow in soil bacteria in the DGGE bands are changes in the performance of these changes with the brightness of the relocation of specific bands and shortcomings of the bands.2. Soil bacterial communities were examined by culture-independent means, namely, 16SrDNA-nested PCR-DGGE profiling. Crude DNA was extracted from soil used to grow different plants, amplified with bacterial specific primers of 16SrDNA by PCR, and separated by denaturing gradient gel electrophoresis(DGGE).Subsequently, bands on DGGE gel were cloned sequenced and the resultant information was used to infer the diversity of bacteria in different soil. The results showed that the same area of soil samples from the DGGE map of the zone and uniformity of the distribution are different. At the same time in different seasons on soilbacteria have an impact on the community composition.