Diversity Of PIF-like Transposable Elements In Bambusoideae Subfamily And The Cloneing Of PIF-like Transposable Elements In Phyllostachys Pubescens

Transposable elements are some DNA fragments which can move and copy to new positions.It was found firstly by American B.McClintock in maize in 1948. The obvious characters are two terminal inverted repeats.Actived Transposons especially long terminal repeats or miniature inverted-repeat transposable elements can insert genes or gene regulation regions which destroy the function of genes or cause the rearrangement of chromosomes. P instability factor (PIF) is an active DNA transoposable element in maize found by chance, which shares similar features with a maize Tourist-like Miniature-inverted repeat transposable elements (MITEs) called miniature PIF (mPIF).It is a significant method by PIF to know the origin and transposable mechanism of MITE.Bamboo especially Mao Bamboo as an mainly all-purpose resource of forest,They play an important role in economy and the development of society. Analyseing the diversity of PIF- like TPase in Bamboo and cloneing the long fragments in Mao Bamboo can lay the foundation for knowing its mechanism.In the future, we will research how is the function of PIF-like TPase during the growth of Mao Bamboo.And get the information whether is the PIF-like TPase related with changes in shape of Mao Bamboo.We also wish PIF-like TPase can be the new tools in genes clone and heredity of biology.In this experment,We selected 44 species representing 38 genera of 6 tribes as testing materials.By PCR amplifications we got 139 distinct PIF-like TPase sequences. All the PIF -like TPase sequences are comprised of 120 amino acids approximatetly presented the majority of the catalytic domain in PIF-like TPases. By polymorphic analysis of the base sequences of PIF-like TPases fragment from bamboo, we found that bamboo transposase sequences are very polymorphic and sequences from two relatively distant species can be more similar to each other than those from two sibling species or from the same genome. So we conclude from these data that PIF-like transposase sequences are monophyletic and extremely heterogeneous. These results can be explained by postulating the existence of multiple ancestral lineages of transposases that diversified early in evolution and were vertically inherited in Bambusoideae subfamily.We conclude from these data that PIF-like transposase sequences are monophyletic and extremely heterogeneous. These results can be explained by postulating the existence of multiple ancestral lineages of transposases that diversified early in evolution and were vertically inherited in Bambusoideae subfamily.In order to learn the configuration of PIF-like transposase, we got its long fragments by constructing a library DNA of mao bamboo. Then useing Southern Blotting to get positive clones.By analysis the positive clones,we got three PIF-like TPase long fragments and the longest sequence was 553 aa.But in the sequences,we found there were many termination codon .It means the TPase long fragments that we got are inactive.