Optimization Of Processing Parameters For Extraction And Meathod Of Quantificational Of Soluble Grifola Frondosa β-Glucan

Systematic studies on the extraction,quantificational,purification and structural characterization of Grifola frondosa polysaccharides and itsβ-glucan were carried out in the presented dissertation.Some new methods and points were developed on the basis of thesis of these studies.The results and creative points are described as follows:1.The result showed curdlan,1,3/1,6-β-glucan of yeast and Grifola frondosa 1,3-β-glucan could bind to Aniline Blue specifically,when the content of the protein was the half of the content of 1,3/1,6-β-glucan,there was no effect on quantificational with the standard 1,3/1,6-β-glucan of yeast.The protein no effect on quantificational with the curdlan standard.Starch,the dextrin,glucose and cellulose no effect. 2.To contrast to detecting method about 1,3-β-glucan about Limulus factor G method and fluorescence method,the result show fluorescence method was simple and feasible.3.By response surface methodology,the optimum polysaccharides conditions of ultrasonic and enzyme assisted extraction was that ultrasonic power was 137.6 W,ultrasonic time was 16.9 min;the optimumβ-glucan conditions was ultrasonic power was 148.3 W. ultrasonic time was 15.6 min.Hydrolyzed temperature was 40℃. hydrolyzed time was 45 min,pH was 6.0 and the quantity of papain was 1.5%of raw material,then extraction was twice under 95℃.By the conditions,the extraction rate of polysaccharides andβ-glucan were 12.75%and 3.60%.4.Separated the crudeβ-glucan by anion exchange and gel chromatographic,then to analyze the homogeneous sugar and structure of aβ-glucan from Grifola frondosa by hot water through High Performance Liquid Chromatography(HPLC),Gas Chromatography (GC),Infrared spectroscopy(IR)and Nuclear Magnetic Resonance (NMR).The innovations that originally studies are embodied in the following several points:1.A simple detecting method about 1,3-β-glucan by fluorescence method established according to some scientific reports. 2.To found the optimum conditions of ultrasonic and enzyme assisted extraction by hot water.3.Determinated of Grifola frondosa 1,3-β-glucan by hot water,cold alkali and hot alkali.4.To analyze structure of aβ-glucan from Grifola frondosa by hot water through GC,IR,NMR.