Requirement Of Protein, Energy, Calcium, Phosphorus And Fatty Acid In The Diet For Juvenile Pelteobagrus Fulvidraco

The optimum protein requirement and energy/protein ratio for Pelteobagrus fulvidraco juvenile(mean body weight,4.7±0.6g) were determined using practical diets in a 62 days trial.Three protein levels(35%,40%and 45%) and two energy levels(15 MJ.kg^-1 and 16 MJ.kg^-1) were tested.Results show that,based on ratio of weight gain, feed conversion ratio,protein efficient rate and condition factor,the optimum diet protein content for juvenile Pelteobagrus fulvidraco is 41.47%～43.38%,optional energy/protein ratio is 40.17.When fed with the diet containing 40.38%of protein and 16.22 MJ.kg^-1 energy,the juvenile got the highest weight gain ratio(132.93%),protein efficiency ratio(1.14) and condition factor(176%),and the lowest feed conversion ratio(2.17).The optimum requirement of calcium and phosphorus and calcium/phosphorus ratio in diets for Pelteobagrus fulvidraco juvenile(mean body weight,4.38±0.69g) were determined using practical diets in a 64 days trial.Four phosphorus levels(1.3%,1.6%, 1.9%and 2.2%) and two calcium levels(2.5%and 3.0%) were tested.Results show that the optimum content of phosphorus in the diet is 1.67%～1.78%,the effects of the content of calcium and calcium/phosphorus ratio to weight gain ratio and feed conversion ratio are unconspicuous.The highest weight gain ratio(295%) were observed in the juvenile fed with the diet containing 1.67%phosphorus,and the lowest feed conversion ratio(1.95) were obtained in the group of the diet with 1.78%phosphoru content.The diet containing 40%of protein and 8%of lipid with total eicosapentaenic acid (EPA) and docosahexaenic acid(DHA)levels(expressed by percentage in total fatty acid level) and n-3/n-6 ratio(adjusted by supplemented with different levels of fish oil,peanut oil,corn oil and sesame oil ) of 1.507,0.178;6.801,0.568;11.402,0.827;17.887,1.224; 22.054,1.838 respectively.The fish fed the dietⅢ(containing 11.402%of EPA and DHA in total lipid and n-3/n-6 ratio 0.827) had the maximal WG(135.83%)and PER(155.20%),while dietⅠ,dietⅢand dietⅣ(with percentage of EPA and DHA in total lipid and n-3/n-6 ratio of 1.507,0.178;11.402,0.827;17.887,1.224 respectively),had lowest FC(1.57,1.63,1.69),.With increase in percentage of EPA and DHA in total lipid and n-3/n-6 ratio,moisture and n-3 fatty acid levels and n-3/n-6 ratio in muscle,gut,and hepapancrase increased while the protein and n-6,n-9 fatty acid levels decreased.The fish fed feed containing 18C:2n-6/18:3n-3 ratio of 8～9 and 8%dietary lipid composed of 53.8%peanut oil, 35.0%corn oil,11.2%sesame oil or 41.2%fish oil,33.8%peanut oil,7.5%corn oil, 17.5%sesame oil,had the maximal WG and PER.