| Genetic analysis on mutative traits and screening of SSR molecular marker were analysised on acceptor 79266 and 04D893 which was produced by introducing Chinese scholar-tree DNA into 79266.The main results of the experiment as follows:1. Many diversity were existed between 79266 and 04D893 in agricultural characters.(1) The diversity in qualitative characterThe abloom type of 79266 was continuously, but that of 04D893 were alternate.The endopleura color of the 79266 was yellow, that of the 04D893 were white.(2) The diversity in quantitative characterThe main stem height,the length of the first pair of lateral branches,pod number per plant,the total pod weight per plant of 79266 were 51.38cm,60.32cm,21.38,41.89g, 04D893 were decreased by 36.36%,22.93%,57.91% and 32.20%,respectively as compared with that of the 79266.The single pod weight,the length of pod shell,the width of pod shell,the thickness of pod shell and eights of perkernel of 79266 were 2.61g,4.10cm,1.64cm,1.40mm,0.93g, 04D893 were increased by 31.15%,12.93%,12.20%,100% and 29.03%,respectively as compared with that of the 79266.The main stem height and the length of the first pair of lateral branches of 04D893 is extremely significant lower than that of 79266;pod and seed kernel of 04D893 is extremely significant higher than that of 79266.2. Heritability evaluation and correlation analysis on mutative traits of 04D893 Broad-sense heritability of 9 quantitative character from high to low is the length of the first pair of lateral branches 76.83%,single pod weight 75.54%,the width of pod shell 73.1%,weights of perkernel 71.05%,main stem height 67.32%,the thickness of pod shell 48.24%,the total pod weight per plant 47.10%,pod number per plant 18.80% and the length of pod shell 18.26%.The results showed that the total pod weight per plant was very significantly correlated with four traits by correlation analysis of 9 quantitative character.The total pod weight per plant has a negative correlation with main stem height, but not significant.3. The characteristics of mutative traits in F2 groups.8 traits showed normal distribution in F2 groups,kurtosis and skewness of other traits were less than 1 except that the length of nut shell was 2.1620,and we can treat those trait except the length of nut shell as quality traits to analysis.4. Optimization of SSR-PCR reaction system of peanut.Optimized SSR system for peanut was established:MgCl2 1.56 mM, dNTP 0.2 mM,Taq 0.043U,template DNA 2 ng/μl,primers 0.5 mM were added to the PCR mixture.Reaction Proeedure Parameters:(l)Pre-denatured DNA at 94℃for 5min,1 cycle (2) denatured DNA 94℃for 40 sec,annealed primer at 53℃for 40 sec,extended primers at 72℃for l min,35 cycles;(3)extended at 72℃for 10 min,1 cycle:(4)Stored at 4℃.5. The screening of polymorphism primers between 04D893 and 79266The results showed that 8 SSR primer pairs were found to produce 18 bands,and 10 were polymorphic between 04D893 and 79266, On average,each primer pair produced 1.25 polymorphic bands in a total of 2.3 bands.6.The QTL analysis of mutative traits in peanutsThe linkage maps was constructed included 8 markers and 2 linkage groups by Mapmaker/Exp(Version3.0).Only 3 QTLS were detected for 8 agronomic traits by CIM,which explained 2%-41% of trait variance. The DSM proved that maker P191 and maker P33 were notable correlation to the germinatingratio with the total pod weight per plant,the length of pod shell and the width of pod shell,maker P177 was notable correlation to the germinatingratio with the length of pod shell. |
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