Identifying The S-genotypes And Analysing Of Genetic Diversity On Sweet Cherry (Prunus Avium L.) Varieties

2The plantation of sweet cherry is one of the most economic benefit fruit industries, and known as"golden industry". Sweet cherry is worth to planting because its fruit is not only fresh and beautiful, but also nutritional. While the correct evaluation and rational utilization on sweet cherry cultivars has been limited because of the miscellaneous name and the self-incompatibility. In order to supply rational collocation for orchards pollination cultivars and theoretical information for evaluation, utilization resources and genetic and breeding research, using 3 pairs of prunus primer combinations to amplify S-allele-specific PCR for 17 kinds of sweet cherry and S-genotypes of sweet cherry cultivars were identified , meanwhile the genetic diversity and relationship were studied by SSR marker technique. The conclusion as follows:1. The effect of PCR amplification with 3 primer combinations is little different. In comparison,the primer combination EM-PC2consFD + EM-PC3consRD is the best one for Sweet cherry, next one is the combination PruC2 +PruC4R, and the last one is the combination PruC2 + PruC5;2. Using the primer combination EM-PC2consFD + EM-PC3consRD to have PCR amplified and the S-gene amplified fragments were cloned and searched in GenBank. As a result, the same S genes display the same size fragments in electrophoretogram. The sizes of S-gene amplification fragments are: S1was 630bp, S2 was 2100bp, S3 was 716bp, S4 was 895bp, S6 was 456bp.3. Combined with three amplification, S-genotype of 17 cultivars were identified, they were showed as follows: 'Dazi' (S₁S₆), 'Tai'anDazi' (S₁S₆), 'Zhifu hong' (S₁S₆), 'Daihong('S₁S₃) , 'Meizao' (S₁S₃), 'Youyi NO.4' (S₁S₃), 'Italy Red'(S₁S₃), 'Zuotengjin'(S₁S₃), 'Jia hong'(S₁S₃), 'Ukraine NO.2'(S₁S₃), 'Hongdeng'(S₁S₃), ' Rainier '(S1 S4), 'Sibake'(S1 S4), 'Hongni('S3 S6), 'Sam('S3S4), 'Napoleon('S3S4), 'Jixin' (S2S4).4. In 20 pairs of SSR primers combinations, nine pairs of them reveal higher polymorphism, such as UDP98-409. The clustering results showed that if the 29 varieties are divided into 5 groups, the genetic diversity is more abundant.