European Plum (prunus Humilison Bunge) Self-incompatibility And The S Gene Cloning

Gametophytic self-incompatibility (GSI) is a widespread mechanism in flowering plants, which prevents self-fertilization and promotes outcrossing. Many fruit frees, such as apple, pear, cherry, aicrot, all display the monofactorial gametophytic self-incompatibility system. Chinese plum-cherry (Prunus humilis Bunge, Rosaceae) is a native of China, which have much value. However, researches on Chinese plum-cherry are still preliminary. Especially on the study about whether Chinese plum-cherry is self-incompatibility or not, research outcomes have seldom been got on published journals up to date. In this work, we confirmed Chinese plum-cherry self-incompatibility, identified as 5-alleles of Chinese plum-cherry. All results were summaried as follows:1. Field pollination test was been done on fifty wild Chinese plum-cherries. The results showed the average fruit set (3.72%) of selfing was much lower than that of cross (>30%), therefore, Chinese plum-cherry displays self-incompatibility.2. SI of Chinese plum-cherry controlled by S-genes was confirmed by field pollination cross test.3. Two pieses of consensus primers, BFP 93 and BFP 94-1, designed from C1 and C2 of Prunus S-RNase, were used with PCR in twelve wild Chinese plum-cherries. After sequenced, the nucleio-acid of seven S-genes were got, which were named S1, S2, S3, S4, S5, S6 and S7, respectively. Then, allele-specific PCR were done with one specific-allele primers and one consensus primer BFP 74. The products of A5-PCR were cloned and the full nucleio-acid sequences of three S-genes (S1, S2 and S3) were obstained successfully. As four S-genes, S4, S5, S6 and S7, have complicated secondary structure and long nucleio-acid sequence, they didn't been amplified and cloned successfully.4. Four S-genotypes of twelve wild Chinese plum-cherries had been identified on the basis of seven S-genes, S1S2, S4S5, S6S7 and S3S7.