Studies On The Properties, Structure Elucidation And Immunological Activity Of The Gum Polysaccharides Isolated And Purificated From Artemisia Sphaerocephala Krasch Seeds

Artemisia sphaerocephala Krasch is widely distributed in desert area of northwest China, and it has potential pharmaceutical value and environment protective functions. In this study, we extracted crude water-soluble and crude water-insoluble gum polysaccharides from Artemisia seeds, respectively. The rheological properties of water-insoluble gum polysaccharides, and the isolation, purification, physic-cochemical properties, structure characterization and immunological activity of crude water-soluble gum polysaccharides were investigated, the results were summarized as follows:1. Extraction crude water-soluble and crude water-insoluble gum polysaccharides from Artemisia seeds, respectively, and isolation, purification and physic-cochemical properties of crude water-soluble gum polysaccharides.The crude water-soluble and crude water-insoluble gum polysaccharides were obtained through water extraction and ethanol precipitation. By column chromatography, two polysaccharides ASPⅠ-A and ASPⅢ-A were purified from crude water-soluble gum polysaccharides. The physic-cochemical properties of ASPⅠ-A and ASPⅢ-A were determined using GC, HPLC, UV and IR (Table 1).2. Studies on the rheological properties of water-insoluble gum polysaccharides from Artemisia seeds (Artemisia seeds gum)The rheological properties of Artemisia seeds gum were studied by viscometer. The results showed the solution of ASG was non-Newtonian fluid (pseudroplastic) . Apparentviscosity decreased with the increase of shear rate, and increased with the increase of concentration. Apparent viscosity had little of change with temperature and pH. The addition of salt affected the apparent viscosity lower. 3. Studies on structure characterization of ASPⅠ-ABased on methylation analysis and partial acid hydrolysis, GC, GC-MS and ESI-MS analysis, ASPⅠ-A was proposed to be a highly branched polysaccharide with a backbone ofβ-1,4-linked-Glucose. The backbone was partially substituted at O-6 of glucosyl residues with mannose, and the non-reducing end were Glc(1- and Gal(1- which were attached to O-4 of mannose. The side chains contained -3)Ara(1-, -2)Glc(1-, -2)Gal(1-, -4)Man(1-, -4,6)Man(1-, Ara(1- and Gal(1-. It was concluded that ASPⅠ-A was a new glucomannan of Artemisia seeds.4. Studies on immunological activity of ASPⅠ-A and ASPⅢ-AWe investigated the effect of ASPⅠ-A and ASPⅢ-A on splenocyte proliferation in mice in vitro. The immunological activity assay revealed that when induced with ConA , ASPⅠ-A and ASPⅢ-A, in a certain range of concentration, could possess a direct effect of mice splenocyte proliferation; ASPⅢ-A may have a certain immunosuppression activity in higher concentration.