Effect Of Artemisia Ordosica Krasch On Antioxidant And Immune Function In Inner Mongolia White Cashmere Goats

The study was conducted to investigate the effects of Artemisia ordosica krasch on antioxidant and immune functions of Inner Mongolia white Cashmere goat.The single factor completely random design was used in this experiment.The forty heathy weanling wether with similar body weights and age?120±10 d?were divided into two groups with4 repetitions.The control group was fed with basal diet and the experimental group replaced the same proportion of roughage with 3%Artemisia ordosica krasch in the basal diet.The trial lasted for 90 days.This study contained three trials.The experiment 1 was conducted to investigate the effects of Artemisia ordosica krasch on blood antioxidant,immune functions and serum biochemical indices of Inner Mongolia white Cashmere goat.The experiment 2 was conducted to investigate the effects of Artemisia ordosica krasch on antioxidant and immune functions of Inner Mongolia White Cashmere Goat tissues?duodenum,liver and spleen?.The experiment 3 was conducted to investigate the effects of Artemisia ordosica krasch on the expression of genes related to antioxidant and immune of tissues to explore the effects through i NOS,Nrf₂ and NF-?B pathway.The results showed as follows:?1?The dietary supplementation with 3%Artemisia ordosica krasch improved T-AOC,CAT and GSH-Px in serum of cashmere goat,decreased the MDA content and test 30-day T-SOD activity on the 90 day,Artemisia ordosica krasch increased the content of NO,i NOS TNF-?,IL-6,IFN-?and IL-2 in serum,but it had no effect on LPS and IL-1?in the serum.?2?The dietary supplementation with Artemisia ordosica krasch reduced NEFA,ALP content and increased TP content in serum.But the content of BUN and GLU of serum decreased and then increased.The effects of Artemisia ordosica krasch on reducing blood sugar and blood lipid of cashmere goat were better at 30 d and 60 d,respectively.?3?Artemisia ordosica krasch promoted the activity of GSH-Px and CAT in liver,spleen and duodenum,the content of TNF-?,NO and iNOS in liver and the secretion of IL-2 and IL-6 in liver and spleen of cashmere goat,but decrease the content of MDA,and increase.?4?The dietary Artemisia ordosica krasch improved the expression of GPx1 in liver and CAT in spleen,IL-1?,IL-2,IL-6,TNF-?,IFN-?,and the expression of Nrf2 and iNOS genes.However,the expression of IL-1?,IL-6 and TNF-?in spleen and NF-?B gene in spleen and duodenum was decreased.?5?In conclusion,the cashmere goat lamb fed the diet containing 3%Artemisia ordosica krasch had higher antioxidant and immune function.The possible mechanism maybe Artemisia ordosica krasch up-regulate the expression of Nrf2 gene,activated the Nrf2 signaling pathway to regulate the expression of GPX1 and CAT genes to increase the activity of antioxidant enzymes.The up-regulation of cytokines expression?such as IL-1??induces the expression of i NOS gene to promote the secretion of NO and inhibits the NF-?B signaling pathway to down-regulate the gene expression of inflammatory factors such as IL-1?,IL-6 and TNF-?in spleen and regulate the secretion of NO.