Study On Effect Of Restoring Lines By Space Mutation,γ-Ray And Combined Treatment

Restoring line in hybrid rice seeds R207 and R198,that were treated by space mutation,⁶⁰Co-γray and combined,were used as materials for experiments.The character expression of SP₁,SP₂ generation and the variance analysis and mutation frequency of SP₂ generation were studied.It studied that the effect of space mutation treatment in mutation frequency and POD activity,SOD activity,CAT activity of restoring line in hybrid rice seeds.After SP₃,SP₄ generation have gotten stability, Choosing some better variations hybridized with sterile rice which has different genotype and the source of cytoplasm,finally we choose the best compages.To some extent,the variation characters of the variations will be express in its hybrid offspring.Therefore,it is an effective way to improve the variation of agronomic characters and economic characters of hybrid offspring through space mutation and radialization mutation to change the characters of restoring line.At the same time,it provide definite academic basic for produce practice of the space mutation -hybrid rice.The main research results as follows:1.Through observing and researching the growth of the plant dealed with Space Mutation's and its variation of agronomic characters and economic characters,we found that the seeds which dealed with space mutation andγ-ray mutation have higher mutant frequency than the seeds which dealed with space mutation orγ-ray mutation separately.To some extent,it indicated that the damnification-effection will be aggravated after the compound dealing,the compound dealing of space mutation andγ-ray Mutation has the adding effection.2.Through statisticing the variation-type and amount of SP₂ generation,and calculate all kinds' the variation-type and mutation frequency according to the mutant number divide the research amount of this kind.The data illuminated that the mutant frequency which dealed with the compound dealing of Space Mutation andγ-ray Mutation is largest,the burliness activity,the grainy weight and the single yield of this two kinds SP₂ generation are very diferent and have larger variation modulus. Through analysing the POD activity,SOD activity,CAT activity,we found that there are remarkable difference between the breed which dealed with space mutation andγ-ray Mutation with the breed which not dealed with.3.We respectively choose the only dealed with space mutation plant,the compound dealing of mpace mutation andγ-ray mutation plant and only dealed withγ-ray Mutation plant in the SP₄ generation,then make them hybridize with the Sterile Rice jin A,xin A which has diferent genotype and the source of cytoplasm,it illuminate that the compounding effection of the researching material R207 collocation is better than R198.To some extent,the variation characters of the variations will be express in its hybrid offspring.It show that diferent restoring line materials has different restoring ability,and this mutation is descendible for its offspring.