| Nerve, endocrine and immunity form a complex, wide network, named neuro-endocrine-immune-regulatory-network, which controls the body's various vital movement, maintains homeostasis of the body. When the homeostasis is disrupted, certain parts in the network will respond and do some change in the function, meanwhile, some change occurs in the referred, leading to certain diseases and the emergence of pathological process. Neuro-endocrine-immune disorder is a common feature of all diseases. All the clinical diseases exist a neuro-endocrine-immune disorder. The course of the disease is actually a disorder for homeostatic regulation of nerve-endocrine-immune network, however, among the nervous, endocrine and immune disorders, immune disorders occupy the leading position.Until now, with regard to immunoregulation participated by the central nervous system(CNS), research positions are concentrated in the hypothalamus and other senior center, while the study is less about the medulla oblongata, especially nucleus ambiguus(Amb) in immunoregulation. Amb, belonging to nucleus nervi vagi, is a special nervus visceralis motor nucleus.The Amb lies in oblongata ventrolateral formatio reticularis and involves a variety of neurons, such as adrenergic neuron, cholinergic neuron, peptidergic neuron. The Amb has a widespread nerve touch with CNS and periphery orgns and plays an important role in the neuro-endocrine-immuno-regulatory network.Inflammatory bowel disease (IBD) is a group of unknown etiology, chronic inflammatory diseases involving in the gastrointestinal tract, including chronic nonspecific ulcerative colitis (UC) and Crohn's disease (CD). Currently, people think they are caused by the interaction of many factors, mainly including immune, environmental and genetic factors, among all of these, immunity, especially cytokines produced by activated immune cells plays a vital role in the occurrence and development of IBD. Many research datas show that its pathogenesis is auto-immune abnormality referring to a variety of cytokines, eventually resulting in tissue damage, and it belongs to a kind of autoimmune diseases. The imbalance between pro-inflammatory cytokines and anti-inflammatory cytokines plays an important role in the pathogenesis of IBD. IL-4 and IL-10 are anti-inflammatory cytokines secreted by Th2 cells. IL-4 is produced by Th2 cells, and IL-10 is produced by Th2 cells, macrophages, mononuclear cells. They have wide immuno-regulation role and play a significant role in the regulation of cytokine network.Since the occurrence and development of IBD are closely related to T cells and their cytokines, Amb play an important role in regulating T-cell-mediated immunity. So, whether the links between nucleus ambiguus and IBD exist, and how Amb play the role in IBD. It has not been reported at home and abroad. To discuss the links among IL-4, IL-10, IBD and Amb, we studied the regulatory role of Amb to IL-4, IL-10 from the view of the nerve-endocrine-immune network and the immune homeostasis regulation. Therefore, we made use of TNBS-induced IBD rats as models and the technology of Amb localization, to detect whether acute electric stimulation Amb in IBD rats will influence IL-4, IL-10 mRNA in colon, thymus, spleen and lymphocytes by Real-time PCR and whether acute electric stimulation Amb in IBD rats will influence the concentration of IL-4, IL-10 in serum by ELISA. We found that IL-4 and IL-10 mRNA were obviously increasing in colon, thymus and spleen after the effective electric stimulation Amb of IBD rats in comparison with those sham-stimulated rats and those matching control rats. However, IL-4 and IL-10 mRNA were obviously decreased in peripheral-blood lymphocyte after the effective electric stimulation Amb of IBD rats in comparison with those sham-stimulated rats and those matching control rats.The concentrations of IL-4 and IL-10 in serum were significantly increasing after the effective electric stimulation Amb of IBD rats in comparison with those sham-stimulated and matching control rats. These demonstrate Amb has an positive regulation to IL-4 and IL-10, but has a negative regulation in peripheral-blood lymphocyte in IBD rats. This result reveals that neuro-endocrine-immune regulatory network participate in the occurrence and development of IBD, Amb has a certain regulation to IBD-related cytokines, may mediate the occurrence and development of IBD, and may play an important role in the occurrence and development of IBD. |
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