Studies On The Determinations Of Carbamate Chemicals Pesticide Residues By High Performance Liquid Chromatography And Dispelling Dynamic Of Carbamate Chemicals Pesticide Residues In Artemisia Annual L. And Soils

Artemisia annual L,as one of Chinese herbal medicine,has anti-malarial activity. Modern medical research has proved that Artemisia annual L.contain Artemisinin, that is a sesquiterpene lactone with an endoperoxide bridge.In the past two years,with the increase of the global demand for the treatment of malignant malaria drugs,the World Health Organization(WHO),UNICEF and other authoritative medical management organizations adopt China's artemisinin to treat malaria.The World Health Organization identified artemisinin as the most safe and effective anti-malarial drug.So Artemisia annual L.as a special plant plays an important role in the health of human.With the increase of requirement for Artemisia annual L.,the cultivation of Artemisia annual L have to depend on manpower.To reduce loss from insects, pesticides are applied to Artemisia annual L.Recent years,carbamate chemicals have been used widely in Artemisia annual L.,so it has a risk of contamination of pesticides.Therefore,it is very important to strengthen the study on determination of carbamate chemicals pesticide residues in Artemisia annual L.This paper explored a series of experiment methods such as extraction, purification and analytical techniques in samples.Methods for the determination of carbamate chemicals pesticide residues in Artemisia annual L.and soil were investigated.The analytical procedure involved in ultrasonic extraction,purification using a Florisil SPE(solid phase extraction) column(6mL,1000mg).The elute fraction was blown to driness under the stream of nitrogen and the residues were dissolved in 1.0mL of acetonitrile,followed by separation and quantitative analysis by reverse phase high performance liquid chromatography(HPLC).The chromatographic conditions,including column temperature,mobile phase,flow rate and ultraviolet detection wavelength were investigated.The optimized chromatographic conditions were:analytical column,Agilent ZORBAX C18,250×4.6mm;column temperature,25℃;mobile phase,acetonitrile-water;flow rate,1.0mL/min;Equally elution from 0 min to 20 min at 35%acetonitrile-water,finally equally elution from 20.01 min to 30 min at 95%acetonitrile-water sharply.The average recoveries obtained from Artemisia annual L samples for pirimicarb were 82.8%(spiked at the levels of 0.1 mg/kg),with relative standard deviations of 7.85%.The average recoveries obtained from Artemisia annual L samples for pirimicarb were 89.6%(spiked at the levels of 0.5 mg/kg),with relative standard deviations of 6.91%.The detection limit of the method was 1.8ng.The average recoveries obtained from Artemisia annual L samples for carbaryl were 81.3%(spiked at the levels of 0.5 mg/kg),with relative standard deviations of 7.53%. The average recoveries obtained from Artemisia annual L samples for carbaryl were 89.2%(spiked at the levels of 1.0 mg/kg),with relative standard deviations of 5.88%.The detection limit of the method was 1.8ng for carbaryl.The average recoveries obtained from soil samples for pirimicarb were 107.9% (spiked at the levels of 0.1 mg/kg),with relative standard deviations of 6.75%.The average recoveries obtained from soil samples for pirimicarb were 87.6%(spiked at the levels of 0.5 mg/kg),with relative standard deviations of 4.71%.The detection limit of the method was 1.8ng.The average recoveries obtained from soil samples for carbaryl were 87.3%(spiked at the levels of 0.5 mg/kg),with relative standard deviations of 7.51%.The average recoveries obtained from soil for carbaryl were 93.4%(spiked at the levels of 1.0 mg/kg),with relative standard deviations of 5.78%. The detection limit of the method was 1.8ng for carbaryl.The results show that half-life period ranged for both pirimicarb and carbaryl were from abort 2 to 3 days.As time went on,the residual contents of pirimicarb and carbaryl in the Artemisia annual L and soil decreased.So they are safe to Artemisia annual L and soil when they were used to reduce loss from insects.