Development Of Rapid Detection Products Of Three Types Of Pesticide Residues In Fruits And Vegetables

Over the past decade,the Enzyme inhibition method has become the mainstream rapid detection method for pesticide residues in fruits and vegetables.However,the result of this method can only judge by naked eye.This defect undermines the reliability and accuracy of the detection,and is not conducive to the large data analysis of agricultural product safety monitoring.Therefore,the improvement of the method is of great significance to realize the whole process control of food safety from farm to table.In addition,because the pyrethroid pesticides are not sensitive to cholinesterase,the Enzyme inhibition method could not be applied to the detection of pyrethroid pesticides.At present,the detection of this type of pesticide is mostly single residue testing,far from meeting the market demand.Therefore,it is important to develop a product which can detect pyrethroid pesticide residues to protect the quality and safety of agricultural products.Based on the above background,two kinds of pesticide residue rapid detection products were developed in this study:(1)A new type of test card has been developed,which can be used for the quantitative detection of organophosphorus pesticides and carbamate pesticides.The experimental results show the sensitivity of the new card is closed to traditional cards which were produced by Lvzhou,and the detection time was shorter(8 min).The coincidence rate of Enzyme disk method and Enzyme reagent method was 94% and 98% respectively.On this basis,this research uses data acquisition device and Photoshop software to establish the conversion formula of inhibition rate,which can be used in the next phase of data acquisition.(2)A rapid determination kit for pesticide residues of 8 different pyrethroid pesticides was developed.The experimental results show that the sensitivity of rapid test kit for0.2~0.3 g/mL,a single sample detection time was 4~6 min,the detection cost is 3~5 yuan /times,the intraassay coefficient of variation was 5.69%~7.61%,coefficient of variationbetween groups was 6.57%.The coincidence rate with the instrument(GC-MS)was 97%.