Anaysis On Genetic Diversity Of B. Campestris. L

In this research, the general PCR reaction was used, the factors of PCR reaction were optimized, and the acrylamide gel was adopted in product testing with silver staining methods. SSR-PCR amplification and detection system were established for this study. Five pairs of specific primers were selected from 29 pairs of SSR primer and they were used on 46 Brassica campestris materials for the PCR amplification, the results of its amplification were analyzed by UPGMA clustering to determine their genetic diversity; at the same time, the SSR fingerprint and their genetic relationship of the main 11 varieties (lines) of winter rapeseed Brassica campestris which current produced and bred in Gansu were studied based on SSR primer analysis. The aims of this study was to provide the basis for our Brassica campestris species genetic diversity and in the breeding of the pro-matching, identification of species, the protection of resources. The results are as follows:1. The vast majority of tested materials can be distinguished by the five pairs of specific primers with SSR cluster analysis. 19 allele were detected, varying from 2.0-6.0; genetic diversity index ranged 0.6303-0.8609 with an average of 0.7162.2. The genetic distance of 46 materials was between 0.7742 and 0.0323. Using UPGMA cluster with PowerMarker (v3.25) software, 46 materials were divided into three major groups at 0.2300 threshold, clustering results and the types of materials had great significance. The first category is the Hezheng, Zhangxian; 15 spring materials were clustered in the second group, at the 0.09 threshold it can be divided into four sub-categories: Longyou3, G-1 was as a sub-category respectively, Qingyou 3, Wanyou 11-1 was as a sub-category; Beiyou1, Menyou1, Menyuan , Longyou4, Qingyou241, Liangdang–zhangjia, Wuwei , Nanfeng , Wudu , XiangⅠ041, XiangⅠ058 were clustered in a sub-category. 29-winter materials were in the third category, which can be further divided into two sub-categories: a sub-category included Dadonggen, Qingshui, Wang8, Minxian, D89-1, Ning 7-2, 826-8-9-7-0, 97 -1-1, 975-1; the other sub-categories made up with S03-512443, 9852, Tianyou 1, 8728-1-1-2, Dongquan , Yanyou 2, 74-1, 986, Chenjiazui, Shangdang,813-1-2-1-3, 964,9889, Tianyou 4, 02 C -9, 876, Longyou 6, Tianyou 2, MXW, WYW; 9852, Tianyou 1, 8728-1-1-2, Dongquan, Yanyu2, 74-1, 986 were in the same group; Chenjiazu, Shangdang , 813 -1-2-1-3,964, 9889, Tianyou 4, 02C-9, 876, Longyou 6, Tianyou 2, MXW, WYW were in another group.3. Compared with the testing material pedigree, SSR analysis results were basically consistent with the variety of pedigree. 4. The analysis results of 11 varieties of winter rape which current produced and bred in Gansu showing that the genetic distance of tested materials was between 0.0323 and 0.4516, and they can be divided into two categories: one category is the S03-512443; the other category is the rest 10 varieties (lines) except S03-512443. The second category can be further divided into two sub-categories :one subclass is 74-1, 9852, Yanyou 2, the second sub-class comprised of Longyou 6, MXW, WYW, Tianyou 2, 02 C-9, 9889, Shangdang. This means that genetic relationship of varieties (lines) was close.5. The SSR fingerprint of 11 varieties (lines) was made based on amplification results of six pairs polymorphical SSR primers on 11 varieties of winter rape, which provided a certain basis for the identification of species, protection of the germplasm resources in future.