Construction Of A Bivalent Antifungal Fusion Gene And Preliminary Identification Of Resistance To Fungi Of Transgenic Tobacco Plants

The harm of fungi and weeds is main reason causing the yield reduction and poor quality of crops.Transferring anti-fungal genes and resistant herbicide genes into plants become direct and efficient way to construct anti-fungal and anti-herbicide plants and of that has attracted many researchers' attention for its high bio-safety and long duration.With more and more anti-fungal proteins are found and applied,the problem of multi-gene transforming and expression arise.It is difficult to realize the aim for the lack of the capacity and the number of vector.Gene fusion and constructing bivalent expression vector of monocotyledon,as a new technology,is the most efficiently way to solve this problem.In this study,a fusion gene with trichoderma chitinase gene andβ-1,3-glucanase gene was constructed,and was transferred corresponding expression vectors to tobacco,against fungal disease and reducing the cost of manpower and financial which are spent on weed control.The main results are as follows:1.Used two anti-fungal gene,classⅠchitinase gene and classⅠβ-1,3-glucanase gene constructed two fusion genes.2.Gene of bar was inserted into pBI121,and obtained plant expression vector pBI121-Bar which contained resistant herbicide gene.3.Monocotyledon bivalent expression vector containing the fusion genes was constructed,and named pBI121-Bar/Chi-Glu.In addition,another two vectors containing CHI,GLU,respectively,were constructed as control.4.The vector pBI121-Bar/Chi-Glu was transferred directly to tobacco by agrobacterium mediated method,and the receptor was leaf of tobaco aseptic seedling. Some trans-genetic plants were obtained by selecting with the PPT as selector,and the Bar gene was selecting marker.