| 1-year-old seedlings of Reaumuria Soongorica and Calligonum Mongolicum were used as the research object in this paper,the optimal medium and hormone combination were studied in different culture stage and the key factor effected the rapid propagation of Reaumuria Soongorica and Calligonum Mongolicum, a more perfect technical route was preliminarily established for Reaumuria Soongorica and Calligonum Mongolicum tissue culture, it can provide some technical support for vegetation restoration in drought condition and In vitro conservation of Reaumuria Soongorica and Calligonum Mongolicum.The main experiment results were as the following:1 1-year-old seedlings below 5cm was the best for explants,the part of plant for selecting explants, the stem of plant was the best , the middle of plant was better , the basal of plant was the worst.2 The best explants disinfection process was: 10% 84 disinfection solution immersion explants 30mins,then 75 % alcohol disinfected 4s and 0.15% HgCl2 disinfected 10min,it could be effectively decreased explants contamination and improved the disinfection efficiencily.3 In Reaumuria Soongorica culture stage ,the best initial culture medium was MS+6-BA0.05 mg·L-1+IAA0.01mg·L-1+AC1.0g·L-1,the best Subculture medium was 1/2MS+6-BA0.01 mg·L-1 +2,4-D0.05mg·L-1,under blue light,the best rooting culture medium was 1/2MSG+IAA0.01 mg·L-1,with 20g·L-1sucrose,the best callus induction medium was H+6-BA0.05 mg·L-1,the best callus multiplication medium was 1/2MS+6-BA0.4mg·L-1+2,4-D0.5mg·L-1 +NAA 0.5mg·L-1,the best callus differentiation medium was H+6-BA0.1mg·L-1+2,4-D0.4mg·L-1。4 Rotten soil was the best for Reaumuria Soongorica and Calligonum Mongolicum transplanting in vitro. the survival rate of Reaumuria Soongorica was more than 89.23%,Calligonum Mongolicum was 90.73%.5 Browning was bad in Reaumuria Soongorica initial culture, addition 1.0g·L-1AC in medium can effective reduction browning,in subculture, the bud differentiation effect was obviously under different light qualities, blue light was the best , fluorescence was the worst.6 In Calligonum Mongolicum culture stage, the best initial culture medium was MS+IAA0.1 mg·L-1+6-BA0.02mg·L-1,the best Subculture medium was MS+IAA0.1 mg·L-1+6-BA0.05 mg·L-1, the best rooting culture medium was MS+IAA0.1 mg·L-1,the best callus induction medium was MS+6-BA0.05mg·L-1,the best callus multiplication medium was MS+6-BA0.4 mg·L-1,the best callus differentiation medium was MS+6-BA0.4 mg·L-1+2,4-D0.5 mg·L-1。...
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