Investigation On The Parasitic Nematode Species Of Strawberry In Xiamen And Studies On The Cultivation And Development Of Aphelenchus Avenae Bastian, 1865

Parasitic nematodes of strawberry in the production areas of Xiamen were investigated during 2006～2007.12 species of plant parasitic nematodes belonging to 6 genus,5 families,and 2 orders were collected,observed and identified.Of them 4 new record species were found on strawberry in China(marked with "☆").These species are:Helicotylenchus dihystera(Cobb,1893) Sher,1961Helicotylenchus digonicus Perry in Perry,Darling et Thorne,1959☆Rotylenchus pumilus(Perry in Perry,Darling et Thorne,1959) Sher,1961Pratylenchus penetrans(Cobb,1917) Chitwood et Otifa,1952Tylenchus sp.Aphelenchus avenae Bastian,1865☆Aphelenchus isomerus Anderson et Hooper,1980Aphelenchoides bicaudatus(Imamura,1931 ) Filipjev et Schuurmans,Stekhoven, 1941☆Aphelenchoides confusus Thorne et Malek,1968Aphelenchoides destructor Ma et Liu,2000☆Aphelenchoides goodeyi Siddiqi et Franklin,1967Aphelenchoides sp.This paper reported the reults of the cultivated methods of pure Aphelenchus avenae Bastian,1865 in 3 different fungal disks(Botrytis cinerea,Pestalotia sp., Trihoderma sp.) at 25℃under darkness,and the effects of the 3 fungs on reproduction of the nematodes.A more vast and rapid population of the nematodes increased on Botrytis cinerea than on Pestctlotia sp.and Rihoderma sp..Each disk was inoculated 20 Aphelenchus avenae females.The nematode population increased with increasing culture time.After 2 weeks,the nematodes cultured on Botrytis cinerea raised to 119600～198650,average 147730.The reproductive coefficient of nematodes is 7786.5.Among these 3 fungs,Botrytis cinerea was the best cultural medium for Aphelenchus avenae.The morphology and development of Aphelenchus avena cultured on Botrytis cinerea at 25℃under darkness were observed and illustrated in this paper.Studies indicated that the nematode completed its embryogenesis in 28 to 34h at 25℃and in its life history there were 4 molts,the fist of which occurred within the egg.The life cycle of this species nemtode was completed for 6 days at 25℃.