| Nervous, endocrine and immune form a complex network, named neuro-endocrine-immune regulatory network, which controls the body's various vital movement, maintains homeostasis of the body. When the homeostasis is disrupted, certain parts in the network will respond and do some change in the function, meanwhile, some change occurs in the referred network, leading to certain diseases and the emergence of pathological process. Neuro-endocrine-immune disorder is a common feature of all the diseases. All the clinical diseases have a nerve-endocrine-immune disorder. The disease process is actually a kind of disorder for homeostasis regulation of nerve-endocrine-immune network, however, among the nerve, endocrine and immune disorders, immune disorder is principal.Ambiguous nucleus (Amb), belonging to nucleus nervi vagi,is a special nervus visceralis motor nucleus.The Amb lies in oblongata ventrolateral formatio reticularis and involves a variety of neurons,such as adrenergic neuron, cholinergic neuron, peptidergic neuron. The Amb has a widespread nerve touch with CNS and periphery orgns and plays an important role in T-cell mediated immunity. Above all, the Amb plays a key role in the neuro-endocrine-immuno- regulatory network.Inflammatory bowel disease (IBD) is a group of unknown etiology, chronic inflammatory diseases involving in the gastrointestinal tract, including chronic nonspecific ulcerative colitis (UC) and Crohn's disease (CD). The distribution of the disease is world-wide, and it seriously endangers the health of our people, and recently its incidence rate is increasing. The etiology and pathogenesis of IBD are not very clear yet. The majority of researches suggest that IBD is a kind of autoimmune disease caused by the interaction of many factors.Among all of these, immunity plays a vital role in the occurrence and development of IBD. Many research datas show that its pathogenesis is auto-immune abnormality referring to a variety of cytokines, eventually resulting in tissue damage. The imbalance of Th1/Th2 immune response and the imbalance between pro-inflammatory cytokines and anti-inflammatory cytokines play an important role in the pathogenesis of IBD. IL-2 and IFN-γare pro-inflammatory cytokines secreted by Th1 cells and have wide immuno-regulation role and play a significant role in the regulation of cytokine network..IL-18 is a kind of pro-inflammatory cytokines mainly produced by Th1 cells.TGF-βis a kind of growth factor, which has bidirectional regulatory function and has a wide range of biological effects. Its production is closely related with Th3. They play an important role in the regulation of cytokine network and have a broad immune regulatory role.Therefore, we made use of TNBS-induced IBD rats as models and the technology of Amb localization, to detect whether acute electric stimulation Amb in IBD rats will influence TGF-β,IL-18 mRNA in colon,thymus,spleen and lymphocytes by Real-time PCR and whether acute electric stimulation Amb in IBD rats will influence the concentration of TGF-β,IL-18 in serum by ELISA. We found that the mRNA of IL-18 was obviously increasing in colon,thymus,spleen and peripheral-blood lymphocyte after the effective electric stimulation Amb of IBD rats in comparison with those sham-stimulated rats and those matching control rats, the mRNA of TGF-βwas also obviously increasing in colon,spleen and peripheral-blood lymphocyte after the effective electric stimulation Amb of IBD rats in comparison with those sham-stimulated and matching control rats, but the mRNA of TGF-βwas decreased in thymus. The concentration of TGF-β,IL-18 in serum was significantly increasing after the effective electric stimulation Amb of IBD rats in comparison with those sham-stimulated and matching control rats. This demonstrates Amb has an positive regulation to IL-18, mainly has an has an positive regulation to TGF-β,but has a negative regulation in the thymus in IBD.This result reveals that: neuro-endocrine-immune regulatory network participate in the occurrence ang development of IBD, Amb has a certain regulation to IBD-related cytokines,may mediates the occurrence and development of IBD, and may play an important role in the occurrence ang development of IBD.
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