Study On Tissue Culture And Rapid Propagation Of Lilium Henryi

In this study,we used the bulblet,leaf,flower organ,basal leaf and renewable bulblet of Lilium henryi Baker as explants,which were cultured in MS medium supplemented with different hormones,such as 6-BA,NAA,KT,IAA.We had established the Lilium henryi system by means of callus and adventitious buds induction and studied the major factors,such as explants materials, medium components and training methods which influenced the induction of callus and adventitious buds.The results showed that:1.The bulblet and leaf left had high pollution,so the rate of callus formation was low;floral organs of the ovary,receptacle had meristematic capacity,a high rate of induced differentiation and the number of budding,and the receptacle's induction time and growth conditions were better than the ovary's,but other parts' mortality was very high.basal leaf and renewable bulblet had a large number of base,value-added quickly,a seedling and no vaccine leaveing.So we celected the receptacle,basal leaf and renewable bulblet as the best induced explants.2.Using the receptacle as explant,we found out the best differentiation callus medium was MS+6-BA2.0(Units:mg/L Following the same)+NAA0.2.Differentiation rate and cofficient differentiation ware 96.67%and 3.1.To the basal leaf,we found out the best differentiation callus medium was MS+6-BA0.5+NAA0.5.Differentiation rate and cofficient differentiation ware 66.67% and 2.0,at the seam time,there were more callus and less roots.The buds grew robustly.To the renewable bulblet,the best medium to induce adventitious buds was MS+6-BA0.5+NAA1.0+KT0.2,at this condition the differentiation rate was 100.00%,coefficient differentiation was 3.8,the small seedling shoot fast,robust growth.3.The appropriate medium for proliferation of adventitious buds was MS+6-BA1.0+NAA0.05, where the buds grew well and the proliferation was 3.2.There were more callus,which had large potential of continuing to divide the adventitious buds.The plant height was uniform,bulbs also significantly enlarged.4.Reducing the concentration of macroelement would help to form root,while 1/2MS was the appropriate medium.NAA was better than IAA in promoting root induction,the best concentration was 0.3 mg/L.The best concentration of sugar was 60g/L.Activated charcoal promoted root's elongation.PP₃₃₃(0.5 mg/L) played arrive at establish brachysm's role.Rooting rate was 100%, average root number was 6.6,now the leaf was fleshy,the colour was deep green,many had 2 movies,which provided a guarantee for later transplanting seeding.Synthesized the various factor, the best rooting medium was 1/2MS+NAA0.3+0.SPP₃₃₃+60g/L(sugar).5.The concentration of sugar and CCC affected the expansion of L.henryi bulbs.The medium-MS+6-BA1.0+NAA0.05 which was appended 3.0 mg/L CCC could expand the small bulb more effectively,the average weight of bulb increased 182mg.The concentration of 30～80g/L sugar,which had more effect on dynamiting.In this range,young bulb diameter was enhanced with the increase of sugar concentration.Higher concentration of sugar would inhibit seeding growth.So 80 g/L sugar concentration were more beneficial to expanding of Lilium henryi bulb.