Detection And Analysis Of Commodity Pig Mucosa Antibody

MIS is generally distributed over air tube,enteron,mucosa of Genitourinary,and some adenoid tissue of anacrine gland,is main place of specific immunity function. sIgA of mucosa immune response process is major effect factor of mucosa immune. Mucosa tissue is a large external environment surface,is a place where animal organism and milieu exterieur exchange each other,mucosa is defensive barrier between animal organism and milieu exterieur,as a Mucosal immunesystem or local immunesystem,especially immunological activity ingredients which is happened in air tube,enteron,Genitourinary tract and also much mucosa,for defence effect of organism infected is more and more reconstr to people.This text through the detect the different parts of commodity pig,such as oral cavity,stomach,duodenum,jejunum,ileum,appendix,colon,rectum,nose,windpipe, bronchus,lung,bladder,urine,cornu uteri,body of uterus,neck of uterus,vagina,tear fluid,bile,paristhmicγ-globulin,the content of IgA,IgG,IgM,to reflect topography of organ mucosa antibody,the whole article contain three part,as following:Part 1:overview the system and composition of Mucosal immunity,process of immune response,research advancement,mucosa immune and disease have intimate correlation,the research of Mucosal immunesystem not only increase the new content for traditionary but also offer the thinking and method of research about pathopoiesis mechanism of many disease,the relationship of etiological agent and host,disease diagnose new generation vaccine and precaution praeparatum.Part 2:the establishment of mucosa antibody detection method,sampling and detection.from the establishment of ELISA to now,already gradually mature,ELISA can detect antigen,also can detect antibody,this test prepare mouse against swineγ-globulin and rabbit against swineγ-globulin,the antibody of IgA,IgG,IgM,use horse radish peroxidase;horseradish peroxidase(HRP) to labeling.to set up the method of double-antibody sandwich ELISA,establish double-antibody sandwich ELISA to detect antibody.To detect each collective mucosa position.(each position collect 30 sample product)Part 3:detection result,through detect the antibody of the commodity pigs different parts,draw development change regular curvt of antibody content,detection result indicate:secretory juice in of digestive system,respiratory apparatus,urinary system,genital system,dacryma,bile,tonsil mucosa all generous IgA,IgG,IgM.