| Mature seeds, immature seeds, in-vitro seedlings of Acacia. fimbriata, A. floribunda, A. crassicarpa, A. leptocarpa, A. confusa, A. melanoxylon, A. implexa and A. podalyriifolia were used as initial materials in this experiment, which included the germination of the mature or immature seeds in vitro in several species of Acacia, the optimization of seedlings in vitro propogation in several species of Acacia, the induction and regeneration of callus in several species of Acacia from different parts of the seedlings, the ability of rooting in several species of Acacia, the content changes of endogenous hormones during the rooting procedure in vitro in several species of Acacia. The main results were as follows:1 Seeds germination in vitro in several species of Acacia.The germination rates in vitro were different when the mature seeds of Acacia. fimbriata, A. floribunda, A. crassicarpa, A. melanoxylon, A. implexa and A. confusa were under the pre- treatment of the concentrated sulphurinc acid for 25 min combined with boiling water for 20 min. The germination rate in vitro of A. floribunda reached only 33.3%, the mature seeds of A. confusa could not germinate, mature seeds of other species in Acacia all germinated at higher frequency more than 70%; the germination rate decreased as the storage time increased. AC was faliciated to the germination of immature seeds.2 Optimization in vitro culture in several species of Acacia.The in-vitro seedlings of Acacia. fimbriata, A. floribunda, A. crassicarpa, A. leptocarpa, A. confusa were used as materials in vitro culture in Acacia spp., the obtained results indicated that the growth of the in-vitro seedlings were different when cultured under the same conditions. When the in-vitro seedlings of A. leptocarpa, A. crassicarpa and A. confusa were cultured, the in-vitro seedlings of A. leptocarpa grew well, vitrifiable or semivitrifiable seedlings did not appear, and the roots developed well when they were cultured on the 1/2MS medium supplemented with 0.4 mg·L-1 BA and 2.0 g·L-1 AC and 50 g·L-1 banana homogenate and 20 g·L-1 sucrose; the multiple shoots could be induced with the rate of 79% and the rate of vitrification was controlled on the lowest level when the in-vitro seedlings of A. crassicarpa were cultured on the MS medium supplemented with 3.0 mg·L-1 BA and 0.2 mg·L-1 IAA and 100 ml·L-1 coconut milk; the proliferation coefficient of A. confusa reached a high level on the medium supplemented with 2.0 mg·L-1 BA, 0.5 mg·L-1 IBA, 0.5 mg·L-1 GA3, 1.0 g·L-1 AC. The multiple shoots could be induced from the cotyledonary nodes of A. crassicarpa, A. melanoxylon, A. implexa when they were cultured on the MS medium supplemented with 2.0 mg·L-1 BA and 0.2 mg·L-1 NAA, which suggested that the cotyledonary nodes should be the ideal materials for the induction of multiple shoots.3 The callus induction and regeneration in several species of Acacia.The hypocotyls of Acacia. fimbriata, A. floribunda, A. podalyriifolia, A. confusa and the leaves of A. fimbriata, A. floribunda, A. crassicarpa, A. leptocarpa, A. confusa and the in-vitro seedlings of A. melanoxylon and A. implexa were used for the materials of callus induction and regeneration, it was found that the induction and regeneration of callus were affected by the parts and the genetypes.4 Rooting culture in several species of Acacia.The in-vitro seedlings of Acacia. fimbriata, A. floribunda, A. crassicarpa, A. leptocarpa, A. confusa were used for the materials of rooting. The results showed that culturing on the 1/2MS medium supplemented with 2.0 mg·L-1 NAA was beneficial to the rooting in A. fimbriata, although the number of axial root and lateral root was not the most, the rooting rate was up to 80%; the MS medium supplemented with 2.0 mg·L-1 NAA and 1.0 mg·L-1 ABT1 was good for the rooting in A. floribunda, the rooting rate reached 88% with the most number of axial root and lateral root; the MS medium supplemented with 2.0 mg·L-1 NAA and 1.5 mg·L-1 IBA was helpful to the rooting in A. crassicarpa, the rooting rate was higher than 90% with the more number of axial root and lateral root; on the 1/2MS medium supplemented with 2.0 mg·L-1 NAA the rooting rate was above 92% with the more number of axial root and lateral root in A. leptocarpa; on the 1/2MS medium supplemented with 1.5 mg·L-1 IBA the highest rooting rate in A. confusa was only 32.5%. The ability and potence of rooting were depended on the genetic basis, and the genetype had a great influence on the rooting ability of Acacia spp.5 Establishment of cell suspension in Acacia.The yellowish friable vigorous callus was used as initial materials for the establishment of cells suspension in A. podalyriifolia, and the optimal initial cell density was 0.5 g per bottle . The system of suspension cells in A. podalyriifolia was first established.6 The HPLC detection of endogenous hormones of plantlets in several species of Acacia.The plantlets of Acacia. fimbriata, A. floribunda, A. crassicarpa, A. leptocarpa, A. confusa were used for the materials of the extraction of endogenous hormones, and the HPLC detection method of endogenous hormones was established in Acacia. The detection of the endogenous hormones in several species of Acacia indicated that the concentrations of endogenous hormones were different from several species of Acacia; and the changes of the endogenous hormones were more important than the contents of the endogenous hormones to the rooting procedure.As a whole, the in vitro culture of Acacia had been systematically carried out in this experiment, the system of suspension cells in A. podalyriifolia was first established, which would be of great significance in micropropagation and breeding by biotechnology in Acacia; the HPLC detection method was used for detecting the endogenous hormones from plantlets in several species of Acacia, which would provide a theoretical guidance for the adding of exogenous hormones in vitro in Acacia, in addition provide a theoretical basis for exploring the basic theories in vitro in Acacia such as cell development and redulation in tissue culture.
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