Screening SNPs Of 6 Genes In Wild Soybean In China

Soybean (Glycine.max) is one of the main economic crops. Because high-yielding cultivars dominate production but are relatively few in number and are genetically similar, genetic diversity in these crops is presumed to have declined to alarmingly low levels.The reduction of genetic diversity does not bode well for future genetic gains in crop productivity and could result in broad susceptibility to newly emerging diseases or insect pests, thereby threatening long-term food and feed security. Due to the high degree of genetic diversity, Glycine.soja has become a valuable genetic resource for soybean breeding. In this study, three N-metabolism related genes: Ammonium transporter(AMT1), nitrate transporter2(NRT2), aspartate aminotransferase2(AspAT2); three resistence-related genes:, ABRE Binding Protein 9(ABP9), betaine-aldehyde dehydrogenase(BADH), calcium dependent protein kinase 13(CPK13) were isolated and cloned using the approach of molecular biology combined with bioinformatics, the related information of SNPs were obtained and the results were as the following:1 Using cDNA sequences of some other species as query sequences, the homologous Expressed Sequence Tags(EST) assembled contigs were searched out on TIGR website; the primers were designed according to the contigs sequences, and part of six genes were isolated.2 All the fragment of the each gene were alingned by using Vector NTI software. 2 SNPs were detected in AMT1, 26 in NRT2, 66 in CPK13, 12 in ABP9, 3 in AspAT, and 27 in BADH. Just the same as reported before, the number of SNPs in non-coding regions is doubled that of SNPs in coding regions.3 Statistics were generated by using DnaSP software5.0, theπof AMT1, CPK13, BADH , ABP9, AspAT2, and NRT2 are 0.00240, 0.02603, 0.00220, 0.00046, and0.02129. Theθof AMT1, CPK13, BADH , ABP9, AspAT2, and NRT2 are 0.00111, 0.02087, 0.00759, 0.00396, 0.00198, 0.01269.