| Nowadays,chemical control is the main ways to control pine weevil(Pissodes nitidus). In order to promote trap effects,it is important to study the chemical communication mechanism between P.nitidus and host plant based on chemical ecology and insect ecology. In this paper,the chemical compounds of volatile from different parts of pines and EAG responses of adults to host plant volatiles were tested.Eight kinds of components which have high efficiency to attract P.nitidus were selected.The morphology and ultramicro sensor of antennal sensilla was observed by SEM and TEM.We also protected the pine with environment friendly chemical agents.The results were summarized as follows:1.The volatile were obtained from four parts of pine,their components were analyzed and by GC-MS and then identified.The results showed that 30 kinds of volatiles are identified in the samples of Pinus koraiensis volatiles,which includ terpene,alkyl hydrocarbon,alkene, ketone,alcoholic and furan.22 kind compounds are the same.The components and relative content changed among different parts.Compared to other parts tested,the concentrations of healthy main tip of a-Pinene were the least,while P-Pinene,Camphene,4(10)-Thujene and 3-Carene were the most,Ocimene was more than damaged main tip,less than subordinate tip and cone.P-Myrcene was less than cone,more than damaged and subordinate tip.α-Farnesene andα-Cubebene were detected on feeded tip.D-Limonene was the peculiar species on subordinate tip,Terpilene,β-Terpinyl acetate,α-Bergamotene,α-Caryophyllene andβ-Sesquiphellandrene were detected only in cone.2.We used EAG technology determine the EAG response of pine weevils to 8 volatiles of the host Korean pine,such as S-α-Pinene,R-α-Pinene,β-Pinene,Ocimene,D-Limonene,3-Camphene,3-Carene,β-Myrcene.The concentration were respectively 0.0004,0.004,0.04,0.4 and 0.8μmol/μL.The result of electrophysiological assay showed that the receptor organs sensitive to volatile compounds,there were no differences of EAG responses between males and females.EAG responses of adults to eight compounds were tested.The result showed that EAG response increaseed with the concentration increaseed.3.We used "Y" tube technology determine the behavior response of pine weevils to 8 volatiles of the host Korean pine.The concentration were respectively 0.0004μmol/μL,0.004μmol/μL,0.04μmol/μL,0.4μmol/μL and 0.8μmol/μL.The result showed:P. nitidus could be attractd by S-α-Pinene and R-α-Pinene when the concentration was 0.0004μmol/μL and 0.04μmol/μL;β-Pinene could be attracted at the concentration 0.4μmol/μL and 0.8μmol/μL.Ocimene could be attracted at the concentration of 0.4μmol/μL and 0.04μmol/μL.However,D-Limonene could not be attracted at 0.4 and 0.8μmol/μL. 4.The external morphology of antennal sensilla was observed by SEM and TEM,six types of sensilla were found,such as sensilla Palmate,sensilla Chaetica,sensilla Trichoid, sensilla Basiconic,sensilla Rod-like,sensilla Falcatas.Sensilla Palmate was the particular type of senilla.And the ultrastructure was tested by TEM.Sensilla Palmate,sensilla Chaetica and sensilla Trichoid were all located on the seventh flagemllum,sensilla Basiconic were on scape and pedicel,sensilla Rod-like were on pedicel and flagemllum,sensilla Falcatas located on scape and the beak.There are many tiny pores on the circumjacent wells of sensilla chaetica and its olfactrory sensilla.5.For the effective prevention and treatment of red pine as Dioryctria hazards,the use of pollution-free pharmaceutical indoor and in forest machine control.The results showed that:in doors,the hypertonic Fenoxycarb,hunting and capture and eliminate aphid mortality crisis more than 50%control more than 70%,and with other control agents compared to a significant difference.Prevention and control in the forest,in order to compare the pharmaceutical high-and low-concentration effects and continuous effect of pharmaceutical.We used Beta-cypermethrin ,Abamectin,Acetamiprid microphone and Acetamiprid microphone,Fenoxycarb hypertonic,Benzoate benzoate streptozotocin Microemulsion at the concentration of 5X,10X and 15X for the prevention of pine weevil.The effect of pharmaceutical compared with the control were significant difference(P<0.01),the concentration of the pharmaceutical were no significant difference(P>0.05).The effects of Abamectin were reach 79.7%-90.7%in the first year,the second year were 98.7%.The second year of continuous control could significantly reduce the rate of Korean pine victims,which was less than 4.0%of the victims. Recommend Avermectin Fenoxycarb and 15X for prevention and treatment to the forest...
|
PDF Full Text Request